用于检测人类 SARS-CoV-2 血清转化的血清学检测方法。
A serological assay to detect SARS-CoV-2 seroconversion in humans.
机构信息
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
出版信息
Nat Med. 2020 Jul;26(7):1033-1036. doi: 10.1038/s41591-020-0913-5. Epub 2020 May 12.
Abstract
Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.
摘要
在这里，我们描述了一种血清酶联免疫吸附试验，用于筛选和鉴定人类 SARS-CoV-2 的血清转化者。该试验不需要处理感染性病毒，可以调整以检测血清和血浆中的不同抗体类型，并适合规模化。血清学检测对于帮助确定人群中以前对 SARS-CoV-2 的暴露、鉴定恢复期血浆治疗的高反应性人类供体以及研究保护相关性非常重要。
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