跨人类和小鼠鉴定与视网膜母细胞瘤相关的微小RNA-信使核糖核酸调控网络和途径。
Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse.
作者信息
Tian Rui, Zou He, Wang Lu-Fei, Song Mei-Jiao, Liu Lu, Zhang Hui
机构信息
Department of Ophthalmology, the Second Hospital of Jilin University, Changchun 130000, Jilin Province, China.
出版信息
Int J Ophthalmol. 2020 Apr 18;13(4):535-544. doi: 10.18240/ijo.2020.04.02. eCollection 2020.
AIM
To explore the mRNA and pathways related to retinoblastoma (RB) genesis and development.
METHODS
Microarray datasets GSE29683 (human) and GSE29685 (mouse) were downloaded from NCBI GEO database. Homologous genes between the two species were identified using WGCNA, followed by protein-protein interaction (PPI) network construction and gene enrichment analysis. Disease-related miRNAs and pathways were retrieved from miR2Disease database and Comparative Toxicogenomics Database (CTD), respectively.
RESULTS
A total of 352 homologous genes were identified. Two pathways including "cell cycle" and "pathway in cancer" in CTD and enrichment analysis were identified and seven miRNAs (including hsa-miR-373, hsa-miR-34a, hsa-miR-129, hsa-miR-494, hsa-miR-503, hsa-let-7 and hsa-miR-518c) were associated with RB. miRNAs modulate "cell cycle" and "pathway in cancer" pathways regulating 13 genes (including CCND1, CDC25C, E2F2, CDKN2D and TGFB2).
CONCLUSION
These results suggest that these miRNAs play crucial roles in RB genesis through "cell cycle" and "pathway in cancer" pathways by regulating their targets including CCND1, CDC25C, E2F2 and CDKN2D.
目的
探讨与视网膜母细胞瘤（RB）发生发展相关的mRNA及信号通路。
方法
从NCBI GEO数据库下载微阵列数据集GSE29683（人类）和GSE29685（小鼠）。使用加权基因共表达网络分析（WGCNA）鉴定两个物种之间的同源基因，随后构建蛋白质-蛋白质相互作用（PPI）网络并进行基因富集分析。分别从miR2Disease数据库和比较毒理基因组学数据库（CTD）中检索与疾病相关的miRNA和信号通路。
结果
共鉴定出352个同源基因。在CTD和富集分析中鉴定出两条信号通路，包括“细胞周期”和“癌症信号通路”，并且有7种miRNA（包括hsa-miR-373、hsa-miR-34a、hsa-miR-129、hsa-miR-494、hsa-miR-503、hsa-let-7和hsa-miR-518c）与RB相关。miRNA调节“细胞周期”和“癌症信号通路”，调控13个基因（包括CCND1、CDC25C、E2F2、CDKN2D和TGFB2）。
结论
这些结果表明，这些miRNA通过调控包括CCND1、CDC25C、E2F2和CDKN2D在内的靶标，在RB发生过程中通过“细胞周期”和“癌症信号通路”发挥关键作用。
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