Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes (LEAMER), Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Programa de Pós-Graduação em Bioquímica (PPGBq), Instituto de Química (IQ), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Biofouling. 2020 Mar;36(3):308-318. doi: 10.1080/08927014.2020.1759558. Epub 2020 May 13.
In the present study, the composition of the extracellular matrix (ECM) of the biofilm formed by , and on a polystyrene surface was investigated. Confocal laser scanning microscopy revealed a dense mycelial mass, with an ECM covering/interspersing the fungal cells and containing carbohydrate-rich molecules (e.g. glycoproteins) and extracellular DNA. The ECMs that were chemically extracted from mature biofilms formed by each of these fungi was predominantly composed of polysaccharides, followed by proteins, nucleic acids and sterols. In general, the amount of biofilm ECM was significantly greater in and than in and Corroborating these results, the disarticulation of mature biofilms with enzymes, sodium metaperiodate and chelating agents occurred mainly in and . Collectively, these results have revealed for the first time the composition of the ECM of the biofilms formed by species and the role it plays in their architecture.
在本研究中，我们调查了 在聚苯乙烯表面形成的生物膜的细胞外基质 (ECM) 的组成。共聚焦激光扫描显微镜显示出密集的菌丝体，其 ECM 覆盖/散布在真菌细胞之间，并且含有富含碳水化合物的分子（例如糖蛋白）和细胞外 DNA。从这三种真菌形成的成熟生物膜中化学提取的 ECM 主要由多糖组成，其次是蛋白质、核酸和固醇。一般来说， 和 形成的生物膜 ECM 量明显大于 和 。这些结果得到了证实，用酶、高碘酸钠和螯合剂使成熟生物膜解体主要发生在 和 中。总的来说，这些结果首次揭示了 种形成的生物膜 ECM 的组成及其在其结构中的作用。
