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重新发现一个老对手：优化的分子方法用于提取和测序粉霉（白粉菌目）真菌标本的 DNA 序列。

Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales).

机构信息

Department of Jobs, Agriculture Victoria Research, Regions and Precincts, Bundoora, Australia.

School of Applied Systems Biology, La Trobe University, Bundoora, Australia.

出版信息

PLoS One. 2020 May 13;15(5):e0232535. doi: 10.1371/journal.pone.0232535. eCollection 2020.

DOI:10.1371/journal.pone.0232535
PMID:32401807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7219758/
Abstract

The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two library preparation kits and found the combination of the E.Z.N.A.® Forensic DNA kit for DNA extraction and the NuGen Ovation® Ultralow System library preparation kit was the most suitable for this purpose.

摘要

本研究旨在确定一种可靠的 DNA 提取方案，用于从 VPRI 参考收藏中的 25 年陈 powder 样 mildew 标本中提取 DNA，以生成高质量的序列，从而解决分类系统发育问题。我们测试了 13 种提取方案和两种文库制备试剂盒，发现 E.Z.N.A.®法医 DNA 试剂盒用于 DNA 提取和 NuGen Ovation® Ultralow 系统文库制备试剂盒的组合最适合于这一目的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/e7d484debb21/pone.0232535.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/d8a5a95e92b0/pone.0232535.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/6ce42804caca/pone.0232535.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/c1025d8714e5/pone.0232535.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/e139d42e3c6f/pone.0232535.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/cebcc77ac522/pone.0232535.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/116916550e7f/pone.0232535.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/e7d484debb21/pone.0232535.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/d8a5a95e92b0/pone.0232535.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/6ce42804caca/pone.0232535.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/c1025d8714e5/pone.0232535.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/e139d42e3c6f/pone.0232535.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/cebcc77ac522/pone.0232535.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/116916550e7f/pone.0232535.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5363/7219758/e7d484debb21/pone.0232535.g007.jpg

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