Seethapathy Parthasarathy, Sankaralingam Subbiah, Pandita Deepu, Pandita Anu, Loganathan Kousalya, Wani Shabir Hussain, El-Ansary Diaa O, Sharma Hanoor, Casini Ryan, Mahmoud Eman A, Elansary Hosam O
Department of Plant Pathology, Amrita School of Agricultural Sciences, Amrita Vishwa Vidyapeetham, Coimbatore 642109, India.
PG and Research Department of Botany, Saraswathi Narayanan College, Madurai 625022, India.
J Fungi (Basel). 2022 Jul 29;8(8):798. doi: 10.3390/jof8080798.
Powdery mildew is an omnipresent disease that reduces the yield and quality of pea crops ( L.). To examine the powdery mildew pathogen's morphological, molecular, and genetic diversity, we collected samples of powdery mildew-affected pea crops from ten distinct locations in the Nilgiris district of Tamil Nadu, India. The pathogen was identified morphologically based on anamorphic characters. Molecular identification of isolates was befitted by targeting the internal transcribed spacer (ITS) region of rDNA and specific primers of powdery mildew fungi. The genetic variation between ten different isolates collected from topographically distinct mountainous areas was studied using random amplified polymorphic (RAPD). Based on its morphological characteristics, the powdery mildew fungus presented high similarities to . Molecular characterization of the ITS rDNA of produced 650 bp nucleotides, PMITS (powdery mildew-internal transcribed region) primers produced 700 bp nucleotides, and an specific ITS primer pair amplified and synthesized 560 bp nucleotides. According to the findings, the collected strains exhibited a low level of genetic diversity and only a slight differential in virulence on the host. In the study, isolates from Anumapuram, Emerald Valley, Indira Nagar, and Thuneri showed a greater disease incidence in the natural field conditions and shared the same genetic lineage with other isolates in UPGMA hierarchical cluster analysis based on RAPD markers. There was no evidence of a link between the occurrence of the disease and these grouped populations.
白粉病是一种普遍存在的病害，会降低豌豆作物（L.）的产量和品质。为了研究白粉病病原菌的形态、分子和遗传多样性，我们从印度泰米尔纳德邦尼尔吉里斯区的十个不同地点采集了受白粉病影响的豌豆作物样本。基于无性型特征对病原菌进行了形态学鉴定。通过靶向rDNA的内部转录间隔区（ITS）区域和白粉病真菌的特异性引物，对分离株进行了分子鉴定。利用随机扩增多态性（RAPD）研究了从地形不同的山区采集的十个不同分离株之间的遗传变异。基于其形态特征，白粉病真菌与……表现出高度相似性。对……的ITS rDNA进行分子特征分析，得到650 bp核苷酸，PMITS（白粉病内部转录区域）引物产生700 bp核苷酸，一对特定的ITS引物扩增并合成了560 bp核苷酸。根据研究结果，所采集的……菌株遗传多样性水平较低，对宿主的毒力差异也很小。在该研究中，来自阿努马普拉姆、翡翠谷、英迪拉纳加尔和图内里的分离株在自然田间条件下病害发生率更高，并且在基于RAPD标记的UPGMA层次聚类分析中与其他分离株具有相同的遗传谱系。没有证据表明病害的发生与这些分组群体之间存在关联。
