Dobrzyn Kamil, Kiezun Marta, Szeszko Karol, Kisielewska Katarzyna, Rytelewska Edyta, Gudelska Marlena, Zaobidna Ewa, Bors Kinga, Kopij Grzegorz, Szymanska Karolina, Kaminska Barbara, Kaminski Tadeusz, Smolinska Nina
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Reprod Domest Anim. 2021 Feb;56(2):239-253. doi: 10.1111/rda.13700. Epub 2020 Dec 6.
This study determined the effect of orexin B (OXB) on the porcine endometrial transcriptome during the embryo attachment phase. Microarray analyses of gene ontology (GO), biological pathways, networks and differentially expressed genes (DEG) were performed. Orexin B influenced the expression of 887 genes (fold change > 1.2; p < .05): 620 genes were up-regulated, and 267 were down-regulated. The analysis of the relationship between DEG revealed that OXB interacts with genes linked with processes such as cell hormone binding, regulation of hormone levels, lipid transport, steroid metabolic processes, the apoptotic signalling pathway and the acute inflammatory response, which are pivotal for reproductive success. Orexin B played a bivalent role in the early-pregnant uterus by limiting the pregnancy outcome, promoting embryo development, suppressing the immune system and, consequently, preventing embryo rejection. These findings suggest that OXB could be responsible for the proper course of gestation by adapting litter size to the metabolic status of the maternal organism.
本研究确定了食欲素B（OXB）在胚胎着床期对猪子宫内膜转录组的影响。进行了基因本体论（GO）、生物途径、网络和差异表达基因（DEG）的微阵列分析。食欲素B影响了887个基因的表达（倍数变化>1.2；p<0.05）：620个基因上调，267个基因下调。对差异表达基因之间关系的分析表明，OXB与细胞激素结合、激素水平调节、脂质转运、类固醇代谢过程、凋亡信号通路和急性炎症反应等过程相关的基因相互作用，这些过程对生殖成功至关重要。食欲素B在妊娠早期子宫中发挥着双重作用，通过限制妊娠结局、促进胚胎发育、抑制免疫系统并因此防止胚胎排斥。这些发现表明，OXB可能通过使窝产仔数适应母体的代谢状态来负责妊娠的正常进程。
