Zaobidna Ewa, Kiezun Marta, Dobrzyn Kamil, Szeszko Karol, Rytelewska Edyta, Kisielewska Katarzyna, Gudelska Marlena, Bors Kinga, Kopij Grzegorz, Szymanska Karolina, Kaminska Barbara, Kaminski Tadeusz, Smolinska Nina
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn-Kortowo, Poland.
Theriogenology. 2020 Oct 15;156:205-213. doi: 10.1016/j.theriogenology.2020.07.002. Epub 2020 Jul 4.
This study aimed to determine the effect of orexin B (OXB) on the global expression pattern and the relationships among differentially expressed genes (DE-genes) in the transcriptome of myometrial explants during the early implantation period in the pig (day 15 of pregnancy). The changes in the transcriptome profile of the porcine myometrium were investigated using the Porcine (V2) Two-colour Gene Expression Microarray, 4 × 44. An analysis of the data from the microarray experiment revealed that 1540 DE-genes were affected by OXB, of which 1135 exhibited fold changes (FC) greater than 1.2 (P < 0.05). Among these, 576 genes were up-regulated and 559 genes were down-regulated. Among the affected biological processes in the myometrial tissue, 76 were enhanced and 31 were suppressed. Furthermore, the differential expression of nine genes, related to the regulation of reproductive functions and metabolic homeostasis, was confirmed by quantitative RT-PCR. A functional analysis of the relationships between DE-genes indicated that OXB interacts with the genes involved in the processes such as the inflammatory response, the response to interleukin-6, cytokine receptor activity, the regulation of cell activation, growth factor receptor binding, lipid modification and the steroid metabolic process. An analysis of DE-genes and their functional relationships suggests that OXB could be involved in the mechanisms such as the regulation of cell proliferation and development, inhibition of contractility, regulation of programmed cell death, and the development of blood vessels, all of which facilitate implantation.
本研究旨在确定食欲素B（OXB）对猪早期着床期（妊娠第15天）子宫肌层外植体转录组中基因整体表达模式以及差异表达基因（DE-基因）之间关系的影响。使用4×44的猪（V2）双色基因表达微阵列研究了猪子宫肌层转录组谱的变化。微阵列实验数据分析显示，1540个DE-基因受OXB影响，其中1135个基因的折叠变化（FC）大于1.2（P<0.05）。其中，576个基因上调，559个基因下调。在子宫肌层组织中受影响的生物学过程中，76个过程增强，31个过程受到抑制。此外，通过定量RT-PCR证实了9个与生殖功能调节和代谢稳态相关的基因的差异表达。对DE-基因之间关系的功能分析表明，OXB与参与炎症反应、对白介素-6的反应、细胞因子受体活性、细胞活化调节、生长因子受体结合、脂质修饰和类固醇代谢过程等过程的基因相互作用。对DE-基因及其功能关系的分析表明，OXB可能参与细胞增殖和发育调节、收缩性抑制、程序性细胞死亡调节以及血管发育等机制，所有这些都有助于着床。
