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人胎盘和骨髓间充质干细胞中毒蕈碱受体的生物学行为

Biological behaviors of muscarinic receptors in mesenchymal stem cells derived from human placenta and bone marrow.

作者信息

Alizadeh Yegani Arash, Maytalman Erkan, Kozanoglu Ilknur, Terzi Menderes Yusuf, Aksu Fazilet

机构信息

Hatay Mustafa Kemal University, Department of Pharmacology and Toxicology, Hatay, Turkey.

Alanya Alaaddin Keykubat University, Department of Medical Pharmacology, Antalya, Turkey.

出版信息

Iran J Basic Med Sci. 2020 Jan;23(1):124-132. doi: 10.22038/IJBMS.2019.38582.9151.

DOI:10.22038/IJBMS.2019.38582.9151
PMID:32405354
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7206840/
Abstract

OBJECTIVES

Cells perform their functional activities by communicating with each other through endogenous substances and receptors. Post-translation, stem cells function properly in new host tissue by carrying specific cell surface receptors. We aimed to characterize muscarinic receptor subtypes in mesenchymal stem cells (MSCs) together with osteogenic and adipogenic differentiation markers.

MATERIALS AND METHODS

mRNA levels of 5 muscarinic receptor subtypes ( to ), BMP-6, and during osteogenic and adipogenic differentiation, under the effect of atropine blockade, were measured in MSCs obtained from human fetal membrane (FM) and bone marrow (BM). Additionally, the effect of atropine on differentiation in the 1st, 2nd, and 3rd passages of MSCs, obtained from human FM and BM, were analyzed by RT-qPCR.

RESULTS

mRNA levels increased in the FM group, while decreasing in the BM group. We found significant decreases in and mRNA levels in FM and BM groups, respectively. Atropine had variable effects based on cell source and receptor type. mRNA levels in differentiated osteogenic cells increased significantly compared to undifferentiated cells in both FM and BM groups. In MSCs derived from both sources, mRNA levels in differentiated adipogenic cells increased significantly. Atropine showed no effect on MSCs differentiation.

CONCLUSION

These results indicate that expressions of muscarinic receptors in MSCs derived from BM and FM can vary and these cells keep the potential of osteogenic and adipogenic differentiation . Besides, atropine had no effect on adipogenic and osteogenic differentiation of MSCs.

摘要

目的

细胞通过内源性物质和受体相互通讯来执行其功能活动。翻译后，干细胞通过携带特定的细胞表面受体在新的宿主组织中正常发挥功能。我们旨在鉴定间充质干细胞（MSC）中与成骨和成脂分化标志物一起的毒蕈碱受体亚型。

材料与方法

在从人胎膜（FM）和骨髓（BM）获得的间充质干细胞中，测量5种毒蕈碱受体亚型（至）、骨形态发生蛋白-6（BMP-6）和成脂分化过程中在阿托品阻断作用下的mRNA水平。此外，通过RT-qPCR分析阿托品对从人FM和BM获得的间充质干细胞第1、2和3代分化的影响。

结果

FM组mRNA水平升高，而BM组降低。我们分别发现FM组和BM组和mRNA水平显著降低。阿托品根据细胞来源和受体类型有不同的作用。与FM组和BM组未分化细胞相比，分化的成骨细胞中mRNA水平显著升高。在来自两种来源的间充质干细胞中，分化的成脂细胞中mRNA水平显著升高。阿托品对间充质干细胞分化无影响。

结论

这些结果表明，来自BM和FM的间充质干细胞中毒蕈碱受体的表达可能不同，并且这些细胞保持有成骨和成脂分化的潜力。此外，阿托品对间充质干细胞的成脂和成骨分化没有影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/f6a3ff5e7a48/IJBMS-23-124-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/2f240379f423/IJBMS-23-124-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/5be81d5489ea/IJBMS-23-124-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/04736ca54ee8/IJBMS-23-124-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/f05f7f834ed3/IJBMS-23-124-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/c8ae47d9bde5/IJBMS-23-124-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/e0ae3f9a1486/IJBMS-23-124-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/f6a3ff5e7a48/IJBMS-23-124-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/2f240379f423/IJBMS-23-124-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/5be81d5489ea/IJBMS-23-124-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/04736ca54ee8/IJBMS-23-124-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/f05f7f834ed3/IJBMS-23-124-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/c8ae47d9bde5/IJBMS-23-124-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/e0ae3f9a1486/IJBMS-23-124-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5352/7206840/f6a3ff5e7a48/IJBMS-23-124-g007.jpg

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