Lee Choong-Hyun
Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 31116, Republic of Korea.
Iran J Basic Med Sci. 2020 Feb;23(2):202-205. doi: 10.22038/IJBMS.2019.38712.9177.
Nuclear receptor-related protein 1 (Nurr1), one of immediate-early genes, is a member of orphan nuclear receptor family. The aim of this study was to investigate the time-dependent change of Nurr1 protein expression in the mouse hippocampal CA3 region following kainic acid (KA)-induced excitotoxic neuronal damage.
Male ICR mice were used as experimental animals, and 30 mg/kg KA was administered intraperitoneally. To confirm the KA-induced neuronal damage in the hippocampal CA3 region, Fluoro-Jade B histofluorescence staining was performed. In addition, the time-dependent change of Nurr1 protein expression was also examined using immunohistochemistry and western blot analysis.
Marked neuronal damage was observed in the hippocampal CA3 region at 24 hr after KA injection. In addition, both Nurr1 immunoreactivity and protein level were significantly increased at 6 hr and 12 hr after KA injection, and then decreased at 24 hr after KA injection.
This result indicates that KA-induced alteration of Nurr1 protein expression may be associated with the neuronal degeneration in the hippocampal CA3 region after KA injection.
核受体相关蛋白1（Nurr1）是即刻早期基因之一，属于孤儿核受体家族。本研究旨在探讨海藻酸（KA）诱导的兴奋性毒性神经元损伤后，小鼠海马CA3区Nurr1蛋白表达随时间的变化。
雄性ICR小鼠作为实验动物，腹腔注射30mg/kg KA。为证实KA诱导的海马CA3区神经元损伤，进行了荧光金B组织荧光染色。此外，还采用免疫组织化学和蛋白质印迹分析检测了Nurr1蛋白表达随时间的变化。
KA注射后24小时，海马CA3区观察到明显的神经元损伤。此外，KA注射后6小时和12小时，Nurr1免疫反应性和蛋白水平均显著升高，然后在KA注射后24小时下降。
该结果表明，KA诱导的Nurr1蛋白表达改变可能与KA注射后海马CA3区的神经元变性有关。
