Pérez-Juárez Angélica, Chamorro Germán, Alva-Sánchez Claudia, Paniagua-Castro Norma, Pacheco-Rosado Jorge
a Department of Pharmacy ;
c Department of Physiology, Escuela Superior De Medicina , Instituto Politécnico Nacional , México , DF , México.
Pharm Biol. 2016 Aug;54(8):1408-12. doi: 10.3109/13880209.2015.1103756. Epub 2016 Jan 22.
Context Arthrospira (Spirulina) platensis (SP) is a cyanobacterium which has attracted attention because of its nutritional value and pharmacological properties. It was previously reported that SP reduces oxidative stress in the hippocampus and protects against damaging neurobehavioural effects of systemic kainic acid (KA). It is widely known that the systemic administration of KA induces neuronal damage, specifically in the CA3 hippocampal region. Objective The present study determines if the SP sub-chronic treatment has neuroprotective properties against KA. Materials and methods Male SW mice were treated with SP during 24 d, at doses of 0, 200, and 800 mg/kg, once daily, and with KA (35 mg/kg, ip) as a single dose on day 14. After the treatment, a histological analysis was performed and the number of atrophic neuronal cells in CA3 hippocampal region was quantified. Results Pretreatment with SP does not protect against seizures induced by KA. However, mortality in the SP 200 and the SP 800 groups was of 20%, while for the KA group, it was of 60%. A single KA ip administration produced a considerable neuronal damage, whereas both doses of SP sub-chronic treatment reduced the number of atrophic neurons in CA3 hippocampal region with respect to the KA group. Discussion The SP neurobehaviour improvement after KA systemic administration correlates with the capacity of SP to reduce KA-neuronal death in CA3 hippocampal cells. This neuroprotection may be related to the antioxidant properties of SP. Conclusion SP reduces KA-neuronal death in CA3 hippocampal cells.
背景 钝顶螺旋藻是一种蓝藻，因其营养价值和药理特性而备受关注。此前有报道称，钝顶螺旋藻可降低海马体中的氧化应激，并预防全身性 kainic 酸（KA）造成的神经行为损伤。众所周知，全身性给予 KA 会导致神经元损伤，尤其是在海马体 CA3 区域。
目的 本研究旨在确定钝顶螺旋藻亚慢性治疗是否对 KA 具有神经保护作用。
材料与方法 雄性 SW 小鼠连续 24 天接受钝顶螺旋藻治疗，剂量分别为 0、200 和 800 mg/kg，每日一次，并在第 14 天单次腹腔注射 KA（35 mg/kg）。治疗后，进行组织学分析，并对海马体 CA3 区域萎缩神经元细胞的数量进行量化。
结果 钝顶螺旋藻预处理不能预防 KA 诱发的癫痫发作。然而，钝顶螺旋藻 200 组和钝顶螺旋藻 800 组的死亡率为 20%，而 KA 组的死亡率为 60%。单次腹腔注射 KA 会造成相当严重的神经元损伤，而钝顶螺旋藻亚慢性治疗的两个剂量组相对于 KA 组均减少了海马体 CA3 区域萎缩神经元的数量。
讨论 KA 全身给药后钝顶螺旋藻对神经行为的改善与钝顶螺旋藻减少海马体 CA3 区 KA 诱导的神经元死亡的能力相关。这种神经保护作用可能与钝顶螺旋藻的抗氧化特性有关。
结论 钝顶螺旋藻可减少海马体 CA3 区 KA 诱导的神经元死亡。
