Wang Yahui, Zhu Yaozhou, Shi Wenzheng, Wang Xichang
College of Food Science and Technology Shanghai Engineering Research Center of Aquatic Product Processing and Preservation Shanghai Ocean University Shanghai China.
Shanghai Totole Food Col, Ltd Shanghai China.
Food Sci Nutr. 2020 Apr 17;8(5):2327-2340. doi: 10.1002/fsn3.1519. eCollection 2020 May.
Total volatile basic nitrogen (TVBN), biogenic amine, total viable count (TVC), volatile compounds, and sensory evaluation were conducted to assess the quality of Chinese Mitten Crabs () at living, zero, 2, 5, 10, 15, and 24 hr postmortem. The sensory evaluation found a noticeable odor of spoilage becoming evident 10 hr postmortem. The TVBN value increased and then decreased as time increased, reaching 23.67 mg N/100 g at 24 hr postmortem. Although biogenic amines were detected at 5 hr postmortem, by 24 hr postmortem these had not reached dangerous levels of toxicity. The initial TVC (6.06 Log CFU/g) of the living crab samples was relatively high and climbed further postmortem, reaching 10.00 Log CFU/g 24 hr postmortem. Trimethylamine was detected in the living sample in belly meat and 2 hr postmortem in crab roe and reached 8.33 µg/g in the roe 24 hr postmortem. Indole was detected at 0 hr (belly meat) and 10 hr (crab roe) postmortem, but did not change significantly during the observation period. Sulfur-containing compounds were detected 5 hr after death and gradually increased over the observation period. Most indicators showed major changes at 5 hr and 10 hr postmortem. By 10 hr postmortem, the crab had entered the putrefaction stage and was thus no longer safe for consumption.
测定了中华绒螯蟹在活体、死后0、2、5、10、15和24小时的总挥发性盐基氮(TVBN)、生物胺、总活菌数(TVC)、挥发性化合物,并进行了感官评价,以评估其品质。感官评价发现,死后10小时明显出现腐败气味。随着时间的增加,TVBN值先升高后降低,死后24小时达到23.67mg N/100g。虽然在死后5小时检测到了生物胺,但到死后24小时,这些生物胺尚未达到危险的毒性水平。活体蟹样本的初始TVC(6.06 Log CFU/g)相对较高,死后进一步攀升,死后24小时达到10.00 Log CFU/g。在活体样本的腹部肌肉中检测到三甲胺,死后2小时在蟹籽中检测到,死后24小时蟹籽中的三甲胺含量达到8.33μg/g。在死后0小时(腹部肌肉)和10小时(蟹籽)检测到吲哚,但在观察期内没有显著变化。死后5小时检测到含硫化合物,在观察期内逐渐增加。大多数指标在死后5小时和10小时出现重大变化。到死后10小时,螃蟹已进入腐败阶段,因此不再适合食用。