从草分枝杆菌中分离并鉴定一种新的非磷酸化脂氨基酸的结构。

Isolation and structural characterization of a new non-phosphorylated lipoamino acid from Mycobacterium phlei.

作者信息

Lerouge P, Lebas M H, Agapakis-Caussé C, Promé J C

机构信息

Centre de Recherche de Biochimie et Génétique cellularie, Toulouse, France.

出版信息

Chem Phys Lipids. 1988 Dec;49(3):161-6. doi: 10.1016/0009-3084(88)90003-5.

DOI:10.1016/0009-3084(88)90003-5

PMID:3240562

Abstract

A new non-phosphorylated lipoamino acid was extracted from Mycobacterium phlei, strain IST. It is particularly sensitive to alkaline hydrolysis, and contains a lysine residue joined to a 1,2-diglyceride via an ester linkage. The FAB-positive mass spectrum shows the presence of various molecular species of which the most abundant contains a palmitic and a tuberculostearic acid residue. An analogue of this lipid was synthesized, 1,2-dipalmitoyl 3-lysyl glycerol. Both its chromatographic behavior (TLC), and the decomposition pathways of the MH+ ions, studied by FAB MS and MIKE spectroscopy, were identical to the natural product.

摘要

从草分枝杆菌IST菌株中提取出一种新的非磷酸化脂氨基酸。它对碱性水解特别敏感，并且含有一个通过酯键与1,2 - 甘油二酯相连的赖氨酸残基。FAB阳性质谱显示存在各种分子种类，其中最丰富的含有一个棕榈酸和一个结核硬脂酸残基。合成了这种脂质的类似物，1,2 - 二棕榈酰3 - 赖氨酰甘油。通过FAB MS和MIKE光谱研究发现，其色谱行为（TLC）以及MH⁺离子的分解途径与天然产物相同。