Couderc F, Aurelle H, Promé D, Savagnac A, Promé J C
Centre de Recherche de Biochimie, CNRS, Toulouse, France.
Biomed Environ Mass Spectrom. 1988 Oct;16(1-12):317-21. doi: 10.1002/bms.1200160161.
The analysis of a complex mixture of monounsaturated fatty acids from mycobacterium phlei is achieved by capillary gas chromatographic separation of their pentafluorobenzyl esters, formation of gas-phase carboxylate anions by electron capture ionization and decomposition of these anions by collision activation. This method allows the structural determination of fatty acid isomers by examination of their collisionally activated dissociation mass-analysed ion kinetic energy spectra. A good correlation is observed between the structure of the unsaturated fatty acids ranging from C24 to C27 and the methyl terminal part of the main chain of diunsaturated mycolic acids.
通过对来自草分枝杆菌的单不饱和脂肪酸复杂混合物的五氟苄酯进行毛细管气相色谱分离、利用电子捕获电离形成气相羧酸根阴离子以及通过碰撞活化分解这些阴离子，实现了对该混合物的分析。该方法通过检查其碰撞活化解离质量分析离子动能谱，能够对脂肪酸异构体进行结构测定。在C24至C27的不饱和脂肪酸结构与双不饱和分枝菌酸主链的甲基末端部分之间观察到良好的相关性。
