Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal, Kharagpur, 721302, India.
Bioprocess Biosyst Eng. 2020 Oct;43(10):1747-1759. doi: 10.1007/s00449-020-02361-1. Epub 2020 May 14.
In this study, Meyerozyma caribbica, an indigenously isolated oleaginous yeast, produced in media containing glucose a bioemulsifier that was partially characterized as a proteoglycan based on preliminary analysis. Optimization of carbon:nitrogen (C:N) ratio revealed 30:1 as the suitable ratio for enhanced production. Apart from higher emulsification activity (E: 70-80%), this molecule showed strong emulsion stability over a wide range of pH (2.0-9.0), salinity (0.05%-10%, w/v) and temperature (- 80 °C to + 50 °C). The current study emphasizes on the determination of critical media parameters for improved and stable bioemulsifier production coupled with partial characterization and identification of the molecule. Thus, a proteoglycan-based bioemulsifier with such a stable emulsifying property can serve as a versatile and potential component in food, cosmetics and pharmaceutical formulations.
在这项研究中，从加勒比海地区分离出来的产油酵母梅里埃毕赤酵母（Meyerozyma caribbica）在含有葡萄糖的培养基中产生了一种生物乳化剂，根据初步分析，该生物乳化剂部分特征为蛋白聚糖。优化碳氮比（C:N）发现 30:1 是提高产量的合适比例。除了更高的乳化活性（E：70-80%）外，这种分子在很宽的 pH 值（2.0-9.0）、盐度（0.05%-10%，w/v）和温度（-80°C 至+50°C）范围内均表现出很强的乳液稳定性。本研究强调确定关键的培养基参数以提高和稳定生物乳化剂的生产，并对该分子进行部分特征描述和鉴定。因此，这种具有稳定乳化性能的基于蛋白聚糖的生物乳化剂可以作为食品、化妆品和制药制剂中的多功能潜在成分。
