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miR-20a/Foxj2 轴通过综合分析鉴定介导结直肠癌细胞的生长和转移。

miR-20a/Foxj2 Axis Mediates Growth and Metastasis of Colorectal Cancer Cells as Identified by Integrated Analysis.

机构信息

Department of General Surgery, Jingmen No. 1 People's Hospital, Jingmen, Hubei, China (mainland).

Department of Burn and Plastic Surgery, Affiliated Nantong Hospital No. 3 of Nantong University, Nantong, Jiangsu, China (mainland).

出版信息

Med Sci Monit. 2020 May 14;26:e923559. doi: 10.12659/MSM.923559.

DOI:10.12659/MSM.923559
PMID:32406388
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7247419/
Abstract

BACKGROUND MicroRNAs (miRNAs) have a significant regulatory effect on the proliferation, migration, and invasion of cells, and have been widely reported to have oncogenic or tumor-suppressive impacts on various tumors. In the present study we assessed the regulation and function of miR-20a on colorectal cancer (CRC) cell lines. MATERIAL AND METHODS qPCR was used to quantify miR-20a expression. Luciferase reporter assay was conducted to confirm Foxj2 3'UTR associations. In addition, the function of miR-20a and Foxj2 in CRC was detected using MTT, colony formation, transwell assays, and cell cycle analysis. RESULTS Our data revealed that miR-20a expression was elevated in the CRC cell lines, and cell migration, proliferation, and invasion abilities were promoted by the overexpression of miR-20a. Moreover, Foxj2 was authenticated as a direct target gene of miR-20a in CRC cells. Furthermore, we found that the ectopic Foxj2 dramatically suppressed miR-20a-promoted proliferation, migration, invasion, and xenografts in vitro and in vivo, and induced cell cycle arrest at G1 stage. CONCLUSIONS Our results showing the roles of miR-20a/Foxj2 in carcinogenesis of CRC may help improve treatment of CRC.

摘要

背景

MicroRNAs (miRNAs) 对细胞的增殖、迁移和侵袭具有重要的调节作用，并且已经广泛报道它们对各种肿瘤具有致癌或肿瘤抑制的影响。在本研究中，我们评估了 miR-20a 对结直肠癌细胞系的调节和功能。

材料和方法

qPCR 用于定量 miR-20a 的表达。荧光素酶报告基因实验用于确认 Foxj2 3'UTR 的关联。此外，使用 MTT、集落形成、transwell 分析和细胞周期分析检测 miR-20a 和 Foxj2 在 CRC 中的功能。

结果

我们的数据显示，miR-20a 在 CRC 细胞系中的表达升高，miR-20a 的过表达促进了细胞迁移、增殖和侵袭能力。此外，Foxj2 被确认为 CRC 细胞中 miR-20a 的直接靶基因。此外，我们发现异位 Foxj2 可显著抑制 miR-20a 促进的体外和体内增殖、迁移、侵袭和异种移植，并诱导细胞周期停滞在 G1 期。

结论

我们的研究结果表明 miR-20a/Foxj2 在 CRC 发生中的作用可能有助于改善 CRC 的治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/487fc133a729/medscimonit-26-e923559-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/251b3a62e1f7/medscimonit-26-e923559-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/2c7cdb636767/medscimonit-26-e923559-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/49419e8a1129/medscimonit-26-e923559-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/487fc133a729/medscimonit-26-e923559-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/251b3a62e1f7/medscimonit-26-e923559-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/b199b811770b/medscimonit-26-e923559-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/2c7cdb636767/medscimonit-26-e923559-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/49419e8a1129/medscimonit-26-e923559-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0c/7247419/487fc133a729/medscimonit-26-e923559-g005.jpg

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本文引用的文献

1
miRTarBase update 2018: a resource for experimentally validated microRNA-target interactions.miRTarBase 更新 2018：一个经过实验验证的 microRNA-靶标相互作用的资源库。
Nucleic Acids Res. 2018 Jan 4;46(D1):D296-D302. doi: 10.1093/nar/gkx1067.
2
The Emerging Roles of Forkhead Box (FOX) Proteins in Osteosarcoma.叉头框（FOX）蛋白在骨肉瘤中的新作用
J Cancer. 2017 Jun 3;8(9):1619-1628. doi: 10.7150/jca.18778. eCollection 2017.
3
Effects of FOXJ2 on TGF-β1-induced epithelial-mesenchymal transition through Notch signaling pathway in non-small lung cancer.
miR-17-92a-1簇宿主基因：结直肠癌发生发展中的关键调节因子。
Clin Exp Med. 2024 Apr 25;24(1):85. doi: 10.1007/s10238-024-01331-1.
4
The REASON score: an epigenetic and clinicopathologic score to predict risk of poor survival in patients with early stage oral squamous cell carcinoma.REASON评分：一种用于预测早期口腔鳞状细胞癌患者生存不良风险的表观遗传学和临床病理评分。
Biomark Res. 2021 Jun 5;9(1):42. doi: 10.1186/s40364-021-00292-x.
FOXJ2对非小细胞肺癌中转化生长因子-β1通过Notch信号通路诱导的上皮-间质转化的影响
Cell Biol Int. 2017 Jan;41(1):79-83. doi: 10.1002/cbin.10680. Epub 2016 Nov 18.
4
Cancer statistics in China, 2015.《中国癌症统计数据 2015》
CA Cancer J Clin. 2016 Mar-Apr;66(2):115-32. doi: 10.3322/caac.21338. Epub 2016 Jan 25.
5
MicroRNA biogenesis pathways in cancer.癌症中的微小RNA生物合成途径。
Nat Rev Cancer. 2015 Jun;15(6):321-33. doi: 10.1038/nrc3932.
6
The role of FoxJ2 in the migration of human glioma cells.FoxJ2在人胶质瘤细胞迁移中的作用。
Pathol Res Pract. 2015 May;211(5):389-97. doi: 10.1016/j.prp.2015.01.005. Epub 2015 Jan 21.
7
miRDB: an online resource for microRNA target prediction and functional annotations.miRDB：一个用于微小RNA靶标预测和功能注释的在线资源。
Nucleic Acids Res. 2015 Jan;43(Database issue):D146-52. doi: 10.1093/nar/gku1104. Epub 2014 Nov 5.
8
miRNA and cancer; computational and experimental approaches.微小RNA与癌症；计算方法和实验方法
Curr Pharm Biotechnol. 2014;15(5):429. doi: 10.2174/138920101505140828161335.
9
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10
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