Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.
Department of Gastroenterology,Jiangxi Province Hospital of Integrated Chinese and Western Medicine, Nanchang, Jiangxi 330003, China.
Biosci Rep. 2020 May 29;40(5). doi: 10.1042/BSR20194345.
Nonalcoholic fatty liver disease (NAFLD) is a frequent metabolic disease and has been demonstrated to contribute to the severity of acute pancreatitis (AP). The present study aimed to investigate the aberrant expression of microRNA-192-5p (miR-192-5p) in AP patients with NAFLD, and further analyze the clinical significance and biological function of miR-192-5p in AP progression.
Expression of miR-192-5p was estimated using quantitative real-time PCR (qRT-PCR). Diagnostic value of miR-192-5p was evaluated by the receiver operating characteristic curve (ROC). The effects of miR-192-5p on cell proliferation, apoptosis and inflammatory response of pancreatic acinar cells were further assessed by CCK-8 assay, flow cytometry and enzyme-linked immunosorbent assay (ELISA).
Circulating miR-192-5p was decreased in AP patients with NAFLD compared with those patients without NAFLD and healthy controls (P<0.05). The down-regulated expression of miR-192-5p had a relative high diagnostic accuracy to distinguish the AP patients with NAFLD from the cases without NAFLD. Furthermore, the overexpression of miR-192-5p in pancreatic acinar cells led to the decreased cell proliferation, increased cell apoptosis and suppressed inflammatory reaction (all P<0.05).
Collectively, all data indicated that serum expression of miR-192-5p in AP patients with NAFLD is significantly decreased and serves as a candidate diagnostic biomarker. The up-regulation of miR-192-5p in pancreatic acinar cell leads to increased cell apoptosis and decreased inflammatory response, suggesting the potential of miR-192-5p as a therapeutic target of AP.
非酒精性脂肪性肝病（NAFLD）是一种常见的代谢性疾病，已被证实与急性胰腺炎（AP）的严重程度有关。本研究旨在探讨 miR-192-5p 在伴有 NAFLD 的 AP 患者中的异常表达，并进一步分析 miR-192-5p 在 AP 进展中的临床意义和生物学功能。
采用实时定量 PCR（qRT-PCR）检测 miR-192-5p 的表达。采用受试者工作特征曲线（ROC）评估 miR-192-5p 的诊断价值。通过 CCK-8 检测、流式细胞术和酶联免疫吸附试验（ELISA）进一步评估 miR-192-5p 对胰腺腺泡细胞增殖、凋亡和炎症反应的影响。
与无 NAFLD 的 AP 患者和健康对照者相比，伴有 NAFLD 的 AP 患者循环 miR-192-5p 降低（P<0.05）。下调 miR-192-5p 的表达对区分伴有和不伴有 NAFLD 的 AP 患者具有较高的诊断准确性。此外，在胰腺腺泡细胞中过表达 miR-192-5p 可导致细胞增殖减少、细胞凋亡增加和炎症反应抑制（均 P<0.05）。
总之，所有数据表明，伴有 NAFLD 的 AP 患者血清中 miR-192-5p 的表达明显降低，可作为候选诊断生物标志物。miR-192-5p 在胰腺腺泡细胞中的上调导致细胞凋亡增加和炎症反应减少，提示 miR-192-5p 可能成为 AP 的治疗靶点。
