Department of Forensic Medicine, Wroclaw Medical University, 4 J. Mikulicza-Radeckiego Street, 50-345, Wrocław, Poland.
Institute of Toxicology Research, 45 Kasztanowa Street, 55-093, Borowa, Poland.
J Forensic Sci. 2020 Sep;65(5):1784-1790. doi: 10.1111/1556-4029.14454. Epub 2020 May 14.
We present results of our study on the stability of 4-chloromethcathinone (4-CMC) in authentic postmortem peripheral blood and vitreous humor samples. The stability of 4-CMC was determined in postmortem blood samples (for a period of 90 days) and vitreous humor (30 days) at three different temperatures: -15°C, +4°C, and + 23°C. The analyses were carried out using ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS). In both materials, the lowest 4-CMC stability was demonstrated at room temperature. The blood samples stored in a freezer (-15°C) showed stability for the entire study period (90 days), while in the case of the vitreous humor sample stored at the same temperature the concentration of the substance decreased by 53% after 30 days. The study carried out in authentic postmortem blood and vitreous humor samples confirms the previous reports of 4-CMC instability in biological material. Authors suggest that the biological material should be stored frozen until analyses are carried out as soon as possible after collection of the material.
我们呈现了关于 4-氯甲卡西酮（4-CMC）在真实死后外周血和玻璃体液样本中稳定性的研究结果。在三个不同温度下（-15°C、+4°C 和 +23°C），我们在死后血液样本（90 天）和玻璃体液（30 天）中测定了 4-CMC 的稳定性。分析采用超高效液相色谱-串联三重四极杆质谱联用（UHPLC-QqQ-MS/MS）进行。在这两种材料中，室温下的 4-CMC 稳定性最低。储存在冰箱（-15°C）中的血液样本在整个研究期间（90 天）均显示出稳定性，而在相同温度下储存的玻璃体液样本中，物质的浓度在 30 天后下降了 53%。在真实的死后血液和玻璃体液样本中进行的研究证实了之前关于 4-CMC 在生物材料中不稳定的报告。作者建议，应将生物材料冷冻储存，直到在收集材料后尽快进行分析。
