Gynecology and Obstetrics Clinic Narodni Front, Kraljice Natalije 62, Belgrade, Serbia.
Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia.
Clin Biochem. 2020 Jul;81:34-40. doi: 10.1016/j.clinbiochem.2020.05.003. Epub 2020 May 11.
The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia.
The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of high-density lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L.
The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856).
AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.
本研究旨在探讨孕早期血脂代谢和氧化应激参数的变化，并更具体地评估研究参数在预测子痫前期中的意义和强度。
本研究纳入了 87 名高危孕妇（HRG）女性，其中 14 名发生了子痫前期（PEC），43 名健康孕妇作为年龄匹配的对照组（CG）。我们测量了丙二醛（TBARS）浓度、脂质过氧化物（LOOH）、促氧化剂抗氧化剂平衡（PAB）和总氧化状态（TOS）作为氧化应激标志物，同时测量了总抗氧化能力（TAC）作为抗氧化防御参数。血浆致动脉粥样硬化指数（AIP）计算为血浆甘油三酯（TG）浓度与血浆高密度脂蛋白胆固醇（HDL-C）浓度之比的以 10 为底的对数，每个浓度均以 mmol/L 表示。
结果表明，HRG 和 PEC 孕妇在孕早期的血脂指标，尤其是 AIP，均显著升高（p<0.001）。HRG 组和 PEC 组的氧化应激参数显著升高，而 TAC 显著降低[0.7±0.15 与 1.1±0.16；（p<0.001）]和[0.6±0.12 与 1.1±0.16；（p<0.001）]与 CG 相比。此外，在 HRG 中，AIP 与子痫前期的发展呈独立相关（p<0.05），而胎盘生长因子未显示出预期的显著水平（p=0.648）。接受者操作特征（ROC）曲线分析表明，研究模型中包含的某些参数对子痫前期具有很好的诊断准确性（AUC=0.856）。
AIP 与高危妊娠相关。此外，我们的结果有力地强调了 AIP 作为子痫前期预测的潜在标志物。
