Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Giza, Egypt.
Microbiology and Immunology Department, Faculty of Pharmacy, October 6 University, Giza, Egypt.
Food Chem. 2020 Oct 1;326:126993. doi: 10.1016/j.foodchem.2020.126993. Epub 2020 May 6.
Food industries are yielding annually plentiful by-products worldwide, these concomitants could be an excellent source of natural functional ingredients. Hence, the antimicrobial and cytotoxic effects of pomegranate husk were examined and chromatographic analyzes were applied for isolation of the bioactive compounds. Lignans; isohydroxymatairesinol, punicatannin C, flavonoids; phloretin, quercetin glycoside, indolamine; punigratane, and phenolic acid; coutaric acid were identified for the first time from pomegranate pericarp, and their structures were elucidated by spectroscopic analysis. Compared to isolated compounds, which showed a weak cytotoxic effects, punicatannin C induced a moderate cytotoxic activity against HepG2 and MCF-7 cell lines. Phloretin and coutaric acid exhibited potent antimicrobial activity againstStaphylococcus epidermidis,while punigratane possesses the most substantial antimicrobial effect onMicrococcus kristinae. In conclusion, pomegranate agrifood by-product; phloretin, punigratane, and coutaric acid display remarkablemicrobicideeffects and could be used in medical, cosmetic, and food industries purposes as a safe, and economical alternative to synthetic agents.
食品工业每年在全球范围内都会产生大量的副产品，这些副产品可能是天然功能性成分的绝佳来源。因此，研究了石榴皮的抗菌和细胞毒性作用，并应用色谱分析分离生物活性化合物。首次从石榴果皮中鉴定出木脂素；异羟基芝麻素、石榴单宁 C、类黄酮；根皮素、槲皮苷、吲哚胺；punigratane 和酚酸；鞣花酸，并通过光谱分析阐明了它们的结构。与显示出弱细胞毒性的分离化合物相比，石榴单宁 C 对 HepG2 和 MCF-7 细胞系表现出中等的细胞毒性活性。根皮素和鞣花酸对表皮葡萄球菌表现出很强的抗菌活性，而 punigratane 对微球菌表现出最强的抗菌作用。总之，石榴农产品副产物；根皮素、punigratane 和鞣花酸具有显著的杀菌作用，可在医学、化妆品和食品工业中用作安全、经济的合成剂替代品。
