Department of Chemistry, University of Rhode Island, 140 Flagg Road, Kingston, RI 02881, USA.
Department of Chemical Sciences, Ariel University, 65 Ramat HaGolan St, Ariel 40700, Israel.
Sensors (Basel). 2020 May 12;20(10):2766. doi: 10.3390/s20102766.
High concentrations of certain nutrients, including phosphate, are known to lead to undesired algal growth and low dissolved oxygen levels, creating deadly conditions for organisms in marine ecosystems. The rapid and robust detection of these nutrients using a colorimetric, paper-based system that can be applied on-site is of high interest to individuals monitoring marine environments and others affected by marine ecosystem health. Several techniques for detecting phosphate have been reported previously, yet these techniques often suffer from high detection limits, reagent instability, and the need of the user to handle toxic reagents. In order to develop improved phosphate detection methods, the commonly used molybdenum blue reagents were incorporated into a paper-based, colorimetric detection system. This system benefited from improved stabilization of the molybdenum blue reagent as well as minimal user contact with toxic reagents. The colorimetric readout from the paper-based devices was analyzed and quantified using RGB analyses (via ImageJ), and resulted in the detection of phosphate at detection limits between 1.3 and 2.8 ppm in various aqueous media, including real seawater.
高浓度的某些营养物质，包括磷酸盐，已知会导致藻类的不良生长和低溶解氧水平，从而对海洋生态系统中的生物造成致命的影响。使用比色、基于纸张的系统快速而稳健地检测这些营养物质，对于监测海洋环境的个人和受海洋生态系统健康影响的其他人来说，具有很高的兴趣。以前已经报道了几种检测磷酸盐的技术，但这些技术通常存在检测限高、试剂不稳定以及用户需要处理有毒试剂的问题。为了开发改进的磷酸盐检测方法，将常用的钼蓝试剂纳入基于纸张的比色检测系统中。该系统受益于钼蓝试剂稳定性的提高以及用户与有毒试剂的接触最小化。通过使用 ImageJ 进行 RGB 分析，对基于纸张的设备的比色读数进行了分析和定量，并在各种水介质中检测到 1.3 到 2.8ppm 之间的磷酸盐检测限，包括真实海水。
