Department of Biomedical Engineering, College of Medicine, Kyung Hee University , Seoul 02447, Republic of Korea.
Department of Surgery, College of Medicine, Inje University , Busan 47392, Republic of Korea.
Anal Chem. 2017 Jun 20;89(12):6448-6454. doi: 10.1021/acs.analchem.7b00300. Epub 2017 May 25.
We introduce a label-free biosensing cellulose strip sensor with surface-enhanced Raman spectroscopy (SERS)-encoded bimetallic core@shell nanoparticles. Bimetallic nanoparticles consisting of a synthesis of core Ag nanoparticles (AgNP) and a synthesis of shell gold nanoparticles (AuNPs) were fabricated on a cellulose substrate by two-stage successive ionic layer absorption and reaction (SILAR) techniques. The bimetallic nanoparticle-enhanced localized surface plasmon resonance (LSPR) effects were theoretically verified by computational calculations with finite element models of optimized bimetallic nanoparticles interacting with an incident laser source. Well-dispersed raspberry-like bimetallic nanoparticles with highly polycrystalline structure were confirmed through X-ray and electron analyses despite ionic reaction synthesis. The stability against silver oxidation and high sensitivity with superior SERS enhancement factor (EF) of the low-cost SERS-encoded cellulose strip, which achieved 3.98 × 10 SERS-EF, 6.1%-RSD reproducibility, and <10%-degraded sustainability, implicated the possibility of practical applications in high analytical screening methods, such as single-molecule detection. The remarkable sensitivity and selectivity of this bimetallic biosensing strip in determining aquatic toxicities for prohibited drugs, such as aniline, sodium azide, and malachite green, as well as monitoring the breast cancer progression for urine, confirmed its potential as a low-cost label-free point-of-care test chip for the early diagnosis of human diseases.
我们介绍了一种无标记生物传感纤维素条传感器，具有表面增强拉曼光谱（SERS）编码的双金属核@壳纳米粒子。双金属纳米粒子由核银纳米粒子（AgNP）的合成和壳金纳米粒子（AuNPs）的合成在纤维素基底上通过两步连续离子层吸收和反应（SILAR）技术制备而成。通过对优化后的双金属纳米粒子与入射激光源相互作用的有限元模型进行计算计算，从理论上验证了双金属纳米粒子增强的局域表面等离子体共振（LSPR）效应。尽管进行了离子反应合成，但通过 X 射线和电子分析证实了双金属纳米粒子具有良好分散的覆盆子状和高度多晶结构。低成本 SERS 编码纤维素条具有出色的稳定性、对银氧化的抗性以及高灵敏度和卓越的 SERS 增强因子（EF），达到了 3.98×10 的 SERS-EF、6.1%-RSD 重现性和 <10%的可持续降解性，表明其在高分析筛选方法中的实际应用的可能性，例如单分子检测。这种双金属生物传感条在确定违禁药物（如苯胺、叠氮化钠和孔雀石绿）的水生毒性以及监测尿液中乳腺癌进展方面的显著灵敏度和选择性，证实了其作为低成本无标记即时护理测试芯片用于人类疾病早期诊断的潜力。
