Faculty of Chemistry, Yazd University, Yazd, Iran.
Department of Drug and Food Control, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
J Sep Sci. 2020 Aug;43(15):3045-3052. doi: 10.1002/jssc.202000221. Epub 2020 Jun 4.
In this work, various types of metal-organic frameworks were synthesized, and their affinities toward buprenorphine were evaluated using dispersive solid-phase extraction. The extracted buprenorphine was determined by ultra high performance liquid chromatography-ultraviolet detection system. The highest extraction recovery was observed by employing zeolitic imidazole framework-67. Then, a facile and fast extraction method was designed for the extraction and purification of the target drug. Optimization of the extraction method was carried out by the design of experiment approach. A linearity range of 1-1000 μg/L with the limit of detection of 0.15 μg/L and relative standard deviations (50 μg/L, n = 5) of 3.4% was obtained for standard sample analysis. Under optimized experimental and instrumental conditions, the relative recoveries were in the range of 95 to 111%. Eventually, zeolitic imidazole framework-67 was successfully employed for the extraction and determination of buprenorphine in the biological fluids with satisfactory results.
在这项工作中，合成了各种类型的金属有机骨架，并使用分散固相萃取法评估了它们对丁丙诺啡的亲和力。采用超高效液相色谱-紫外检测系统测定萃取的丁丙诺啡。通过使用沸石咪唑骨架-67 观察到最高的萃取回收率。然后，设计了一种简便快速的萃取方法用于目标药物的萃取和纯化。通过实验设计方法对萃取方法进行了优化。标准样品分析得到的线性范围为 1-1000μg/L，检出限为 0.15μg/L，相对标准偏差（50μg/L，n=5）为 3.4%。在优化的实验和仪器条件下，相对回收率在 95%至 111%之间。最终，沸石咪唑骨架-67 成功用于生物体液中丁丙诺啡的提取和测定，结果令人满意。
