Department of Dermatology, Ajou University School of Medicine, Suwon, Korea; Department of Biomedical Science, The Graduate School, Ajou University, Suwon, Korea.
Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.
J Invest Dermatol. 2020 Dec;140(12):2478-2486.e4. doi: 10.1016/j.jid.2020.04.016. Epub 2020 May 15.
Senescent fibroblasts play a role in aging pigmentation. In this study, we found that GDF15 expression levels are increased in UV-irradiated senescent fibroblasts and photoaged hyperpigmented skin. To investigate the effects of GDF15 on melanogenesis, normal human melanocytes were cocultured with fibroblasts infected with the GDF15 lentivirus or GDF15 short hairpin RNA. It was found that GDF15 stimulates melanogenesis in melanocytes through MITF/tyrosinase upregulation via β-catenin signaling. The stimulatory action of GDF15 during pigmentation was further confirmed in ex vivo cultured skin and in a reconstituted human skin sample. These results suggest that senescent fibroblast-derived GDF15 stimulates skin pigmentation and may play a role in aging-associated pigmentation.
衰老的成纤维细胞在衰老性色素沉着中起作用。在这项研究中，我们发现 GDF15 的表达水平在 UV 照射的衰老成纤维细胞和光老化的色素沉着皮肤中增加。为了研究 GDF15 对黑素生成的影响，将正常人黑素细胞与感染 GDF15 慢病毒或 GDF15 短发夹 RNA 的成纤维细胞共培养。结果发现，GDF15 通过 β-连环蛋白信号通路上调 MITF/酪氨酸酶来刺激黑素细胞中的黑素生成。在离体培养的皮肤和重建的人皮肤样本中进一步证实了 GDF15 在色素沉着过程中的刺激作用。这些结果表明，衰老的成纤维细胞来源的 GDF15 刺激皮肤色素沉着，并可能在与衰老相关的色素沉着中发挥作用。
