Kilpatrick G J, Jones B J, Tyers M B
Department of Neuropharmacology, Glaxo Group Research Ltd., Ware, U.K.
Neurosci Lett. 1988 Nov 22;94(1-2):156-60. doi: 10.1016/0304-3940(88)90287-x.
We have examined the distribution of specific binding of the 5-HT3 receptor ligand [3H]GR65630 to rat brain using quantitative autoradiography. Brain tissue was frozen and thin sections (20 microns) cut. Slide-mounted tissue sections were incubated with [3H]GR65630 (0.2 nM) in the presence or absence of metoclopramide (30 microM, to define non-specific binding). After drying, sections were apposed to photographic film for 3 months. Films were quantified for radioactivity using image analysis. The highest level of specific binding was found in the area postrema (34.0 fmol/mg tissue). Specific binding was high in intermediate layers of the cortex, particularly the entorhinal, temporal and pyriform cortex (5.2-7.0 fmol/mg tissue). Amongst sub-cortical areas, binding was evident in the olfactory lobes, olfactory tubercle, hippocampus and basolateral amygdala (1.3-3.3 fmol/mg tissue). Specific binding was below measurable levels in other sub-cortical areas.
我们使用定量放射自显影技术研究了5-羟色胺3（5-HT3）受体配体[3H]GR65630与大鼠脑组织特异性结合的分布情况。将脑组织冷冻并切成20微米的薄片。将载于载玻片上的组织切片与[3H]GR65630（0.2纳摩尔）一起孵育，同时加入或不加入甲氧氯普胺（30微摩尔，用于确定非特异性结合）。干燥后，将切片与摄影胶片贴合3个月。使用图像分析对胶片的放射性进行定量。在最后区发现特异性结合的最高水平（34.0飞摩尔/毫克组织）。在皮质的中间层，特别是内嗅皮质、颞叶皮质和梨状皮质，特异性结合水平较高（5.2 - 7.0飞摩尔/毫克组织）。在皮质下区域中，嗅叶、嗅结节、海马体和基底外侧杏仁核有明显的结合（1.3 - 3.3飞摩尔/毫克组织）。在其他皮质下区域，特异性结合低于可测量水平。
