伊朗西北部若法勒县一般人群包虫病的血清流行病学研究（IHA、ELISA 和 Western blot，2017-2018 年）。

Sero-epidemiology of Hydatidosis Among General Population of Jolfa County, Northwestern Iran Using IHA, ELISA and Western Blot (2017-2018).

机构信息

Cellular & Molecular Research Center and Department of Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

Cellular & Molecular research Center and Department of Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

出版信息

Infect Disord Drug Targets. 2021;21(2):193-201. doi: 10.2174/1871526520666200516162813.

DOI:10.2174/1871526520666200516162813

PMID:32416709

Abstract

BACKGROUND

Human hydatidosis is mostly a latent and neglected disease with known endemicity in Iran.

AIMS

Due to the importance of this infection in the country and its latent nature, we aimed to evaluate the serological status of hydatid cyst in northwestern Iran.

OBJECTIVES

Herein, we evaluated the serological status of hydatid cyst in urban and rural inhabitants of Jolfa county, northwestern Iran during 2017-2018.

METHODS

In total, 1296 blood samples were obtained from human individuals and the presence of anti-E. granulosus antibodies was investigated using IHA, ELISA and WB.

RESULTS

Based on results, 25 IHA positive person were detected in the examined population, however ELISA test showed 14 of 25 IHA positive patients as negative. Also, 269 IHA negative fellows were shown as negative by ELISA. WB analysis of sera from 25 IHA positive subjects revealed consistent results with the ELISA test, and the most reactive SHCF Ag was a 37 KDa protein. The age-standardized seroprevalence of hydatidosis among Jolfa's general population was 1.12% with 95%CI: 1.02-1.20. Moreover, there existed a significant association between keeping/- contact with dogs (P = 0.022) as well as vegetable consumption (P < 0.001) with ELISA positive test results.

CONCLUSION

Along with such serological evidence in this region, we highly suggest physical examination and applying imaging techniques for suspected cases in the area for a better understanding of CE.

摘要

背景

人体包虫病在伊朗流行，多呈潜伏和被忽视状态。

目的

鉴于该感染在该国的重要性及其潜伏性质，我们旨在评估伊朗西北部人群的包虫囊肿血清学状况。

方法

本研究于 2017 年至 2018 年期间，评估了伊朗西北部 Jolfa 县城乡居民的包虫囊肿血清学状况。共采集了 1296 份人血样本，使用间接血凝试验（IHA）、酶联免疫吸附试验（ELISA）和免疫印迹法（WB）检测抗 E. granulosus 抗体的存在。

结果

在所检查的人群中，根据结果发现 25 例 IHA 阳性者，然而 ELISA 检测显示 25 例 IHA 阳性患者中有 14 例为阴性。此外，ELISA 检测还显示 269 例 IHA 阴性者为阴性。对 25 例 IHA 阳性患者血清进行 WB 分析，结果与 ELISA 检测结果一致，最具反应性的 SHCF Ag 是 37 kDa 蛋白。Jolfa 人群的包虫病年龄标准化血清流行率为 1.12%，95%CI：1.02-1.20。此外，与狗接触/饲养（P = 0.022）以及食用蔬菜（P < 0.001）与 ELISA 阳性检测结果之间存在显著关联。