子痫前期孕妇外周血和胎盘组织中 IL-17/IL-35 的表达失衡。

Expression imbalance of IL-17/IL-35 in peripheral blood and placental tissue of pregnant women in preeclampsia.

机构信息

Department of Obstetrics and Gynecology, Clinical Medical College of Yangzhou University, Yangzhou, 225001, Jiangsu Province, China.

出版信息

Taiwan J Obstet Gynecol. 2020 May;59(3):409-414. doi: 10.1016/j.tjog.2020.03.013.

DOI:10.1016/j.tjog.2020.03.013

PMID:32416889

Abstract

OBJECTIVES

The possible mechanism of preeclampsia is investigated in this study to facilitate the exploration of the future remediation of this disease by analysing the changes of IL-17 and IL-35 in peripheral blood and placental tissue of pregnant women with preeclampsia (PE).

MATERIALS AND METHODS

The study was conducted using 45 healthy pregnant women as the control group and 90 pregnant women in the preeclampsia group, including 45 cases with severe preeclampsia and 45 cases with mild preeclampsia. All of 135 pregnant women underwent caesarean delivery. IL-17 and IL-35 concentrations in the serum were measured by ELISA, and IL-17 and IL-35 expression in placental specimens was detected by immunohistochemistry.

RESULTS

There were no statistically significant differences in age among the three study groups. Serum IL-17 levels were significantly higher in PE patients than in healthy pregnant women (P < 0.01). The ratio of positive staining for IL-17 was markedly higher in mild PE tissues (84.44%; 38/45) and severe PE tissues (86.67%; 39/45) than in healthy pregnant tissues (35.56%; 16/45) (P < 0.01). The strong positive rates for IL-17 were markedly higher in mild PE tissues (48.89%; 22/45) and severe PE tissues (68.89%; 31/45) than in healthy pregnant tissues (13.33%; 6/45) (P < 0.01). No differences between mild PE tissues and severe PE tissues were noted in both positive case rates and strong positive rates. Consistent with this finding, the ratio of strong positive staining for IL-35 was higher in healthy pregnant tissues (66.67%; 30/45) than in mild PE tissues (33.11%; 14/45) and severe PE tissues (26.67%; 12/45) (P < 0.01).

CONCLUSIONS

The abnormal increase in serum and placental of IL-17 has an association with the formation and development of PE. IL-35 expression is significantly lower in severe PE placenta tissue and serum compared with normal pregnant women. These results suggested that IL-17/IL-35 imbalance may play a role in the pathophysiology of PE.

摘要

目的

本研究旨在探讨子痫前期的可能发病机制，通过分析子痫前期患者外周血和胎盘组织中白细胞介素 17（IL-17）和白细胞介素 35（IL-35）的变化，为未来该病的治疗提供参考。

材料与方法

采用病例对照研究，选取 45 例健康孕妇作为对照组，90 例子痫前期孕妇作为病例组，其中重度子痫前期 45 例，轻度子痫前期 45 例，所有孕妇均行剖宫产术。采用酶联免疫吸附法（ELISA）检测血清中 IL-17、IL-35 浓度，免疫组化法检测胎盘组织中 IL-17、IL-35 表达。

结果

三组孕妇年龄比较，差异无统计学意义。子痫前期患者血清 IL-17 水平明显高于健康孕妇（P<0.01）。轻度子痫前期组织（84.44%，38/45）和重度子痫前期组织（86.67%，39/45）IL-17 阳性染色率明显高于健康孕妇组织（35.56%，16/45）（P<0.01）。轻度子痫前期组织（48.89%，22/45）和重度子痫前期组织（68.89%，31/45）IL-17 强阳性率明显高于健康孕妇组织（13.33%，6/45）（P<0.01）。轻度子痫前期组织与重度子痫前期组织的阳性病例率和强阳性率差异均无统计学意义。IL-35 强阳性染色率在健康孕妇组织（66.67%，30/45）高于轻度子痫前期组织（33.11%，14/45）和重度子痫前期组织（26.67%，12/45）（P<0.01）。