纳米羟磷灰石的分散体对体外口腔生物膜的影响。
The effect of disaggregated nano-hydroxyapatite on oral biofilm in vitro.
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Centre for Oral Diseases, Sichuan University, Chengdu 610064, China; Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Centre for Oral Diseases, Sichuan University, Chengdu 610064, China; Department of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
出版信息
Dent Mater. 2020 Jul;36(7):e207-e216. doi: 10.1016/j.dental.2020.04.005. Epub 2020 May 13.
OBJECTIVE
Agglomeration is a common problem facing the preparation and application of nanomaterials, and whether nano-hydroxyapatite (nano HA) can modulate oral microecology left to be unclear. In this study, nano HA was disaggregated by sodium hexametaphosphate (SHMP) and ultrasonic cavitation to observe whether agglomeration would affect its effect on oral bacterial biofilm.
METHODS
Dynamic light scattering (DLS) and scanning electronic microscope (SEM) were used to observe the treatment solutions. Single-species biofilms and multi-species biofilms were treated with 10% nano HA, 10% disaggregated nano HA, 10% micro hydroxyapatite (micro HA) and deionized water (DDW) for 30min and analyzed via MTT assay, lactic acid measurement, SEM and confocal laser scanning microscope (CLSM). Real-time polymerase chain reaction was performed to analyze the biofilm composition.
RESULTS
Ultrasonic cavitation combined with SHMP could significantly reduce the degree of agglomeration of nano HA. Disaggregated nano HA could inhibit bacterial growth and reduce the ability of bacterial biofilm to produce lactic acid and extracellular polysaccharides. There was no significant difference on composition of multi-species biofilms between nano HA and disaggregated nano HA.
SIGNIFICANCE
The disaggregated nano-hydroxyapatite could inhibit the metabolism and acid production of oral bacterial biofilm, but did not significantly affect the composition of multi-species biofilms.
目的
团聚是纳米材料制备和应用中普遍存在的问题，纳米羟基磷灰石（nano HA）是否能调节口腔微生态尚不清楚。本研究通过六偏磷酸钠（SHMP）和超声空化对纳米 HA 进行解团聚，观察团聚是否会影响其对口腔细菌生物膜的作用。
方法
动态光散射（DLS）和扫描电子显微镜（SEM）观察处理液。用 10%纳米 HA、10%解团聚纳米 HA、10%微羟基磷灰石（micro HA）和去离子水（DDW）处理单种生物膜和多种生物膜 30min，通过 MTT 检测、乳酸测定、SEM 和共聚焦激光扫描显微镜（CLSM）进行分析。实时聚合酶链反应分析生物膜组成。
结果
超声空化联合 SHMP 可显著降低纳米 HA 的团聚程度。解团聚纳米 HA 可抑制细菌生长，降低细菌生物膜产酸和产生胞外多糖的能力。纳米 HA 和解团聚纳米 HA 对多种生物膜的组成无显著差异。
意义
解团聚纳米羟基磷灰石可抑制口腔细菌生物膜的代谢和产酸，但对多种生物膜的组成无显著影响。
Zotero 插件