Lebedev K A, Petrov R V, Lopukhin Iu M, Stenina M A, Andreeva O K
Zh Mikrobiol Epidemiol Immunobiol. 1977 Feb(2):130-4.
The authors carried out a complex immunomorphological study of the peripheral blood lymphocytes in 160 healthy persons of different age groups. The tests of spontaneous and complementary rosette formation of lymphocyte blasttransformation with PHA were used, assessing it by the H3-thymidine incorporation, lymphocyte straining by Coon's direct and indirect methods for detection of surface immunoglobulin receptors; G, A, and M immunoglobulins blood serum content was studied by Mancini's method. Mean indices characterizing the T- and B-populations of lymphocytes in persons of different age were calculated on the basis of these studies.
作者对160名不同年龄组的健康人外周血淋巴细胞进行了一项复杂的免疫形态学研究。采用淋巴细胞与PHA进行自发和互补性玫瑰花结形成试验,通过³H-胸腺嘧啶核苷掺入法进行评估,采用库恩氏直接和间接法对淋巴细胞进行染色以检测表面免疫球蛋白受体;用曼奇尼法研究血清中G、A和M免疫球蛋白的含量。基于这些研究计算出不同年龄人群淋巴细胞T亚群和B亚群的平均指标。