Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.
Zoonoses Public Health. 2020 Dec;67(8):849-861. doi: 10.1111/zph.12713. Epub 2020 May 17.
Rotavirus C (RVC) is an enteric pathogen that affects humans and animals around the world.
In this study, we characterized the genetic diversity of RVC strains detected in asymptomatic Brazilian pigs by sequencing the NSP4, NSP5 and VP6 genes.
The results of reverse transcription polymerase chain reaction showed that 53 of 579 samples (9.2%) contained RVC. Positive samples were genotyped by sequencing gene segments NSP4, and NSP5. Most of the RCV strains encountered were classified into typically porcine genotypes: E1-H1. In two strains, BP182 and BP208, the NSP4 gene grouped with E2-RVC human strains with 94.2%-96.5% nucleotide identity, although the NSP5 gene was porcine-like (H1). In strain SD67, the NSP5 gene grouped with human H2-RVC with 92.5%-98.7% nucleotide identity and the NSP4 gene grouped with porcine strains (E1). Two strains (BP208 and SD67) were also genotyped by sequencing gene segment VP6. The VP6 gene grouped with porcine strains, I6 (89.3%-90.2% nucleotide identity) and I5 (88.7%-90.5% nucleotide identity), for strains BP208 and SD67, respectively.
These results are indicative of genomic reassortment between RVC strains of human and porcine origin. In recent years, the incidence of RVC infection among humans has increased significantly. It is important to measure the frequency of interspecies transmission in order to monitor the evolution of these viruses and to identify rearranged strains that may lead to an epidemic.
轮状病毒 C（RVC）是一种肠道病原体，影响着全球的人类和动物。
在本研究中，我们通过测序 NSP4、NSP5 和 VP6 基因，对巴西无症状猪中检测到的 RVC 株的遗传多样性进行了特征描述。
逆转录聚合酶链反应的结果显示，579 个样本中有 53 个（9.2%）含有 RVC。通过测序基因片段 NSP4 和 NSP5 对阳性样本进行了基因分型。遇到的大多数 RCV 株被分类为典型的猪基因型：E1-H1。在 2 株 BP182 和 BP208 中，NSP4 基因与 94.2%-96.5%核苷酸同一性的 E2-RVC 人株分组，尽管 NSP5 基因是猪样的（H1）。在株 SD67 中，NSP5 基因与人 H2-RVC 的核苷酸同一性为 92.5%-98.7%，而 NSP4 基因与猪株（E1）分组。对 2 株（BP208 和 SD67）还通过测序基因片段 VP6 进行了基因分型。VP6 基因与猪株 I6（89.3%-90.2%核苷酸同一性）和 I5（88.7%-90.5%核苷酸同一性）分组，分别用于株 BP208 和 SD67。
这些结果表明，RVC 株的人源和猪源之间存在基因重配。近年来，RVC 感染在人类中的发病率显著增加。测量种间传播的频率对于监测这些病毒的进化和识别可能导致流行的重排株非常重要。
