Kattoor Jobin Jose, Saurabh Sharad, Malik Yashpal Singh, Sircar Shubhankar, Dhama Kuldeep, Ghosh Souvik, Bányai Krisztián, Kobayashi Nobumichi, Singh Raj Kumar
a Division of Biological Standardization , ICAR-Indian Veterinary Research Institute , Bareilly , India.
b Division of Pathology , ICAR-Indian Veterinary Research Institute , Bareilly , India.
Vet Q. 2017 Dec;37(1):252-261. doi: 10.1080/01652176.2017.1346849.
Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog.
To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes.
A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed.
Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population.
The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.
轮状病毒C（RVC）是腹泻暴发的已知病原体，主要感染全球猪群，在人类、牛、雪貂、水貂和犬中偶有发生。
证实印度猪群中存在RVC，并对其选定的结构基因（VP6）和非结构基因（NSP4和NSP5）进行特征分析。
使用来自印度不同地区的108份腹泻样本。将分离的RNA加载到聚丙烯酰胺凝胶上，通过鉴定特定的电泳基因组迁移模式来筛选轮状病毒的存在。为了对RVC毒株进行特征分析，对VP6基因以及NSP4和NSP5基因进行扩增、测序和分析。
基于VP6基因特异性诊断逆转录聚合酶链反应，在12.0%（13/108）的仔猪粪便样本中证实存在RVC。对选定的猪RVC（PoRVC）毒株编码内衣壳蛋白的VP6基因进行核苷酸序列分析，发现与欧亚起源的人类RVC毒株（HuRVC）有超过93%的同源性。这些毒株与迄今报道的PoRVC毒株不同，与HuRVC毒株聚类，属于I2基因型。然而，发现这些毒株的两个非结构基因，即NSP4和NSP5，属于猪型，这表明印度猪群中发生了重配事件。
研究结果表明印度猪中存在类人RVC，并将I2基因型的RVC进化枝进一步分为亚进化枝。据我们所知，这似乎是印度猪群中RVC的首次报道。猪中类人RVC VP6基因的出现支持了其随后的人畜共患病前景。
