CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
J Biomater Sci Polym Ed. 2020 Aug;31(12):1604-1621. doi: 10.1080/09205063.2020.1764191. Epub 2020 May 18.
We report the efforts to construct active targeting quantum dots using receptor-binding peptide for enhanced detection and migration inhibition of cancer cells. Peptide E5 has specific binding with chemokine receptor 4 (CXCR4), which is a transmembrane G-coupled receptor involved in the metastasis of various types of cancers. E5 was introduced to the surface of CdSe/ZnS quantum dots biotin-streptavidin interactions. The constructed CXCR4-targeting quantum dots (E5@QDs) was observed to display improved detection sensitivity and significantly enhanced binding affinity for CXCR4 over-expressed cancer cells, and the ability to inhibit cancer cells migration induced by CXCL12.
我们报告了使用受体结合肽构建主动靶向量子点的努力，以增强对癌细胞的检测和迁移抑制。肽 E5 与趋化因子受体 4（CXCR4）具有特异性结合，CXCR4 是一种参与多种类型癌症转移的跨膜 G 蛋白偶联受体。E5 通过生物素-链霉亲和素相互作用被引入到 CdSe/ZnS 量子点的表面。构建的 CXCR4 靶向量子点（E5@QDs）显示出提高的检测灵敏度，并且对过表达 CXCR4 的癌细胞具有显著增强的结合亲和力，并且能够抑制由 CXCL12 诱导的癌细胞迁移。
