Li Chao, Hu Jiao, Liu Peihua, Li Qiaqia, Chen Jinbo, Cui Yu, Zhou Xu, Xue Bichen, Zhang Xin, Gao Xin, Zu Xiongbing
Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
Xiangya School of Medicine, Central South University, Changsha 410008, China.
Transl Androl Urol. 2020 Apr;9(2):232-242. doi: 10.21037/tau.2020.03.01.
Cystitis glandularis (CG) is a proliferative disorder of the urinary bladder characterized by transitional cells that have undergone glandular metaplasia. The underlying mechanism associated with this transformation is poorly understood.
The expression of messenger RNA (mRNA) and long non-coding RNA (lncRNA) from normal bladder mucosa and CG were compared using microarray analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to describe molecular interactions.
Microarray analysis identified 809 significantly dysregulated mRNAs in CG tissues; 606 were up-regulated and 203 were down-regulated (greater than 2-fold difference in expression from normal tissue, P<0.05). KEGG pathway analysis showed that the mRNAs that co-expressed with lncRNAs were enriched in the cell cycle regulation pathway. Four up-regulated lncRNAs (ENST00000596379, ENST00000463397, NR_001446 and NR_015395) were identified in the coding-non-coding co-expression (CNC) network analysis as being associated with the expression of four mRNAs (SMAD3, ORC1, CCNA2 and CCNB2). NR_015395 was revealed to be a competing endogenous RNA (ceRNA) of miR-133a-3p that targets SMAD3.
This is the first work to measure the expression of dysregulated lncRNA and ceRNA in CG and identify the crosstalk between mRNA and lncRNA expression patterns in the pathogenesis of CG.
腺性膀胱炎（CG）是一种膀胱增殖性疾病，其特征为发生了腺化生的移行细胞。这种转变相关的潜在机制尚不清楚。
使用微阵列分析比较正常膀胱黏膜和腺性膀胱炎中信使核糖核酸（mRNA）和长链非编码核糖核酸（lncRNA）的表达。采用京都基因与基因组百科全书（KEGG）通路分析来描述分子间相互作用。
微阵列分析确定腺性膀胱炎组织中有809个mRNA显著失调；606个上调，203个下调（与正常组织相比表达差异大于2倍，P<0.05）。KEGG通路分析表明，与lncRNAs共表达的mRNA在细胞周期调控通路中富集。在编码-非编码共表达（CNC）网络分析中，鉴定出4个上调的lncRNAs（ENST00000596379、ENST00000463397、NR_001446和NR_015395）与4个mRNA（SMAD3、ORC1、CCNA2和CCNB2）的表达相关。NR_015395被揭示为靶向SMAD3的miR-133a-3p的竞争性内源性RNA（ceRNA）。
这是第一项测量腺性膀胱炎中失调lncRNA和ceRNA表达并确定腺性膀胱炎发病机制中mRNA和lncRNA表达模式之间相互作用的研究。
