Department of Urology, Xiangya Hospital, Central South University, Changsha, China.
J Cell Physiol. 2019 Aug;234(8):13592-13601. doi: 10.1002/jcp.28037. Epub 2019 Jan 4.
Bladder cancer (BCa) is one of the most prevalent cancers of the urinary system worldwide. Accumulating evidence suggests that long noncoding RNAs (lncRNAs) perform a vital function in the pathogenesis and progression of BCa. In the current study, we identified a novel lncRNA OXCT1-AS1 and investigated its role and potential mechanisms in BCa. The microarray results showed the expression of lncRNAs, microRNAs, and messenger RNAs between BCa primary tumor tissues and metastatic lymph nodes were significantly different. The quantitative polymerase chain reaction verification was performed to ensure the reliability of the screening results. The Cell Counting Kit 8 and transwell assay were used to assess the tumor cell proliferation and invasion abilities in vitro, respectively. The dual-luciferase activity assay was performed to investigate the potential mechanism of competing endogenous RNA network. lncRNA OXCT1-AS1, which elevated in metastasis lymph node, was significantly upregulated in BCa cell lines compared with SVHUC-1. We demonstrated OXCT1-AS1 inhibited miR-455-5p to decrease its binding to the JAK1 3'-untranslated region, which could upregulate the expression of JAK1 at the protein level, thus promoting BCa proliferation and invasion. Therefore, lncRNA OXCT1-AS1 could act as a potential biomarker and therapeutic target for patients with BCa.
膀胱癌 (BCa) 是全球泌尿系统最常见的癌症之一。越来越多的证据表明，长非编码 RNA (lncRNA) 在 BCa 的发病机制和进展中发挥着重要作用。在本研究中，我们鉴定了一种新型 lncRNA OXCT1-AS1，并研究了其在 BCa 中的作用和潜在机制。微阵列结果显示，BCa 原发肿瘤组织和转移性淋巴结之间的 lncRNA、microRNA 和信使 RNA 的表达有显著差异。通过定量聚合酶链反应验证来确保筛选结果的可靠性。细胞计数试剂盒 8 和 Transwell 分析分别用于评估体外肿瘤细胞增殖和侵袭能力。双荧光素酶活性测定用于研究竞争内源性 RNA 网络的潜在机制。lncRNA OXCT1-AS1 在转移淋巴结中上调，在 BCa 细胞系中与 SVHUC-1 相比显著上调。我们证明 OXCT1-AS1 抑制 miR-455-5p，减少其与 JAK1 3'-非翻译区的结合，从而上调 JAK1 的蛋白水平，从而促进 BCa 的增殖和侵袭。因此，lncRNA OXCT1-AS1 可以作为 BCa 患者的潜在生物标志物和治疗靶点。
