Department of Chemistry, Graduate School of Science, Tohoku University, Japan, Sendai 980-8578, Japan.
Org Biomol Chem. 2020 Jun 3;18(21):4009-4013. doi: 10.1039/d0ob00794c.
We report on the design of new siRNA-binding fluorescent probes with the improved detection selectivity toward intact double-stranded siRNAs over single-stranded forms by the conjugation of pyrene unit into thiazole orange base surrogate-carrying peptide nucleic acid (PNA) that can simultaneously recognize the 3'-overhang and double-stranded sequences of target siRNAs.
我们报告了新的 siRNA 结合荧光探针的设计，通过将芘单元与能够同时识别靶 siRNA 的 3'-突出端和双链序列的噻唑橙碱基替代物携带肽核酸（PNA）连接，提高了对完整双链 siRNA 的检测选择性，而不是单链形式。
