Lai Fangyuan, Kakudo Natsuko, Morimoto Naoki, Ma Yuanyuan, Kusumoto Kenji
Department of Plastic and Reconstructive Surgery, Kansai Medical University; Department of Plastic Surgery, Zhejiang Provincial People's Hospital.
Department of Plastic and Reconstructive Surgery, Kansai Medical University;
J Vis Exp. 2020 May 4(159). doi: 10.3791/60480.
Activated platelet-rich plasma (PRP) prepared from whole blood via centrifugation demonstrated a proliferation-stimulating effect in several kinds of cultured cells, implying a possible use in regenerative medicine. Here, a double-spin method was used to prepare PRP from whole blood. PRP was further activated by autologous thrombin. The platelet count was measured in the activated PRP and the proliferation-stimulating effect in human adipose-derived stem cells (hASCs) was examined. The resulting platelet count was 11.5-times higher in PRP than in whole blood plasma. The proliferation of hASCs was markedly enhanced by incubation with 1% PRP. The described method can be used to reproducibly prepare PRP with a high concentration of platelets. PRP prepared by this method markedly promotes proliferation of hASCs.
