State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Engineering Research Center of Recycling & Comprehensive Utilization of Pharmaceutical and Chemical Waste of Zhejiang Province, Taizhou University, Jiaojiang 318000, China.
Anal Chem. 2020 Jun 16;92(12):8450-8458. doi: 10.1021/acs.analchem.0c01162. Epub 2020 Jun 1.
Recently emerged liposomal photoelectrochemical (PEC) bioanalysis has brought new opportunities for biosensor development. This work presents the new concept of liposome-assisted enzymatic modulation of plasmonic photoelectrochemistry for PEC bioanalysis, which was exemplified by an Au nanoclusters (NCs)-sensitized nanoporous TiO nanotubes (Au NC@TiO NT) photoelectrode and an alkaline phosphatase (ALP)-loaded liposomal immunoassay of heart-type fatty acid binding protein in a 96-well plate. After sandwich immunorecognition and subsequent lysis treatment, enzymatically generated ascorbic acid by the released ALP was directed to reduce Au into Au nanoparticles using the Au NCs as seeds, leading to the in situ change of the photoelectrochemistry of the electrode and corresponding reduction of the photocurrent. The depressed signal could be correlated with the target concentration with good analytical performance in terms of sensitivity and selectivity. This work features the liposome-assisted enzymatic modulation of plasmonic photoelectrochemistry, which provides a new protocol for general PEC bioanalysis development.
最近出现的脂质体光电化学（PEC）生物分析为生物传感器的发展带来了新的机遇。本工作提出了脂质体辅助的酶调制等离子体光电化学用于 PEC 生物分析的新概念，以 Au 纳米团簇（NCs）敏化的多孔 TiO 纳米管（Au NC@TiO NT）光电极为例，并在 96 孔板中进行了碱性磷酸酶（ALP）负载的脂质体免疫测定人心型脂肪酸结合蛋白。在三明治免疫识别和随后的裂解处理后，释放的 ALP 酶促生成的抗坏血酸在 Au NCs 的作用下被引导还原为 Au 纳米颗粒，导致电极的光电化学原位变化，相应地减少了光电流。抑制信号与目标浓度呈良好的相关性，在灵敏度和选择性方面具有良好的分析性能。本工作的特点是脂质体辅助的酶调制等离子体光电化学，为一般 PEC 生物分析的发展提供了新的方案。
