Hiraoka T, Kihira K, Kosaka D, Kohda T, Hoshita T, Kajiyama G
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Steroids. 1988 May-Jun;51(5-6):543-50. doi: 10.1016/0039-128x(88)90050-5.
Bile alcohol glucuronides present in human serum were isolated by ion exchange chromatography on piperidino-hydroxypropyl Sephadex LH-20. Following hydrolysis with beta-glucuronidase, the bile alcohols were analyzed by a combination of gas-liquid chromatography and mass spectrometry. Bile alcohols identified were 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25,26-hexol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,26-pentol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25,26-pentol, and 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25,26-hexol. The bile alcohol composition in serum was similar to that in urine but not to that in bile. The concentration of total bile alcohols in serum was 59.5 +/- 24.6 micrograms/L.
采用哌啶基-羟丙基葡聚糖凝胶LH-20离子交换色谱法分离人血清中的胆汁醇葡糖醛酸苷。用β-葡糖醛酸苷酶水解后，通过气相色谱和质谱联用分析胆汁醇。鉴定出的胆汁醇有27-降-5β-胆甾烷-3α,7α,12α,24,25-戊醇、27-降-5β-胆甾烷-3α,7α,12α,24,25,26-己醇、5β-胆甾烷-3α,7α,12α,24,25-戊醇、5β-胆甾烷-3α,7α,12α,24,26-戊醇、5β-胆甾烷-3α,7α,12α,25,26-戊醇和5β-胆甾烷-3α,7α,12α,24,25,26-己醇。血清中的胆汁醇组成与尿液中的相似，但与胆汁中的不同。血清中总胆汁醇的浓度为59.5±24.6微克/升。
