Kuwabara M, Ushiroguchi T, Kihira K, Kuramoto T, Hoshita T
J Lipid Res. 1984 Apr;25(4):361-8.
Bile alcohols present in urine from healthy adults were studied. Urine was extracted with octadecylsilane-bonded silica gel, and a neutral fraction and a glucuronide fraction were isolated by ion exchange chromatography on piperidinohydroxypropyl Sephadex LH-20. Following hydrolysis of the glucuronide fraction with beta-glucuronidase and purification by silica gel column chromatography, the bile alcohols were analyzed by a combined gas-liquid chromatography-mass spectrometry. By direct comparison with reference standards, the structures of four major bile alcohols were elucidated as follows: 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25- pentol ; 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24, 25- pentol ; 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25,26- pentol ; and 5 beta-cholestane-3 alpha,7 alpha,12 alpha,26,27- pentol . The daily excretion of these four bile alcohols in urine was 0.5-1.0 mumoles. After purification by silica gel column chromatography, the neutral fraction was analyzed by a combined gas-liquid chromatography-mass spectrometry. The major bile alcohol excreted in urine as the unconjugated form was 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25- pentol . The daily excretion of the unconjugated C26 pentol (0.02-0.05 mumoles) was far lower than that of the conjugated C26 pentol (0.34-0.86 mumoles).
对健康成年人尿液中的胆汁醇进行了研究。尿液用十八烷基硅烷键合硅胶萃取，通过在哌啶羟丙基葡聚糖凝胶LH - 20上进行离子交换色谱法分离出中性部分和葡糖醛酸苷部分。用β - 葡糖醛酸苷酶水解葡糖醛酸苷部分并通过硅胶柱色谱法纯化后，通过气相色谱 - 质谱联用仪分析胆汁醇。通过与参考标准品直接比较，阐明了四种主要胆汁醇的结构如下：27 - 去甲 - 5β - 胆甾烷 - 3α,7α,12α,24,25 - 戊醇；5β - 胆甾烷 - 3α,7α,12α,24,25 - 戊醇；5β - 胆甾烷 - 3α,7α,12α,25,26 - 戊醇；以及5β - 胆甾烷 - 3α,7α,12α,26,27 - 戊醇。这四种胆汁醇在尿液中的每日排泄量为0.5 - 1.0微摩尔。通过硅胶柱色谱法纯化后，用气相色谱 - 质谱联用仪分析中性部分。以未结合形式排泄到尿液中的主要胆汁醇是27 - 去甲 - 5β - 胆甾烷 - 3α,7α,12α,24,25 - 戊醇。未结合的C26戊醇的每日排泄量（0.02 - 0.05微摩尔）远低于结合的C26戊醇的每日排泄量（0.34 - 0.86微摩尔）。
