Ruggiero G, Andreana A, Ninni A, Curci G, Utili R, Giusti G
Acta Hepatogastroenterol (Stuttg). 1977 Apr;24(2):102-5.
Liver efficiency in clearing circulating BCG (Clamette-Guèrin bacillus) was studied using the isolated rat liver. Bacteria were added to the perfusate at a concentration of 1 X 10(6) cells/ml, and the medium was then sampled at subsequent intervals for 6 hours in 2 perfusions and for 1 hour in 7 perfusions. At the end of all perfusions, liver and bile samples were obtained and used for viable bacterial counts. For each perfusion the bactericidal activity which might have been exerted by serum present in the perfusate was also investigated. About 95% of BCG disappeared from the perfusate after 6 hours of perfusions, and 90% after 1 hour. Recovery of viable bacteria in the liver at 60 minutes averaged 80% of the inoculum. Recovery in bile was negligible. Control experiments indicated that extrahepatic factors in possible reduction of bacterial concentration in the perfusate did not interfere with hepatic removal, per se.
使用离体大鼠肝脏研究了肝脏清除循环中的卡介苗（卡迈特-介兰杆菌）的效率。将细菌以1×10⁶个细胞/毫升的浓度加入到灌注液中，然后在2次灌注中随后每隔一定时间对培养基取样6小时，在7次灌注中取样1小时。在所有灌注结束时，获取肝脏和胆汁样本并用于活菌计数。对于每次灌注，还研究了灌注液中存在的血清可能发挥的杀菌活性。灌注6小时后，约95%的卡介苗从灌注液中消失，1小时后为90%。60分钟时肝脏中活菌的回收率平均为接种量的80%。胆汁中的回收率可忽略不计。对照实验表明，灌注液中细菌浓度可能降低的肝外因素本身并不干扰肝脏的清除作用。
