Ruggiero G, Gaeta G B, Andreana A, Galante D
Boll Ist Sieroter Milan. 1978 Mar 31;57(1):23-5.
The clearance of S. typhi Ty 2 and of a S. aureus strain by the isolated rat liver perfused in vitro with a synthetic serum free perfusate was studied. Bacteria were added to the perfusate at the initial concentration of 1x10(8) cells/ml. During perfusions the perfusate was sampled; after 30 min bile and liver samples were obtained and used for viable bacterial counts. After 30 min the amount of bacteria phagocytized greatly differed from one strain to the other: 30% for S. typhi Ty 2 and 100% for S. aureus. The end amount of viable bacteria recovered in the isolated liver was 7% for S. typhi Ty 2 and 80% for S. Aureus, thus indicating that for the latter strain greater clearance was not associated with an equally fast intracellular death.
研究了用无血清合成灌注液体外灌注分离的大鼠肝脏对伤寒杆菌Ty 2和金黄色葡萄球菌菌株的清除情况。将细菌以1×10⁸个细胞/毫升的初始浓度加入到灌注液中。在灌注过程中对灌注液进行采样；30分钟后获取胆汁和肝脏样本并用于活菌计数。30分钟后，不同菌株吞噬的细菌数量差异很大：伤寒杆菌Ty 2为30%，金黄色葡萄球菌为100%。分离肝脏中回收的活菌最终数量，伤寒杆菌Ty 2为7%，金黄色葡萄球菌为80%，这表明对于后一种菌株，更高的清除率并不伴随着同样快速的细胞内死亡。
