Department of Biochemistry, Kharkiv National Medical University, Kharkiv, Ukraine.
Department of Pathological Anatomy, Kharkiv National Medical University, Kharkiv, Ukraine.
Acta Medica (Hradec Kralove). 2020;63(1):18-24. doi: 10.14712/18059694.2020.11.
To evaluate the effects of orally administered gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) on the course of chronic carrageenan-induced intestinal inflammation.
Samples of small intestinal tissue were collected from four groups of rats (intact, after administration of VNPs, with carrageenaninduced intestinal inflammation, with carrageenan-induced intestinal inflammation orally exposed to VNPs) to assess the intestinal morphology and HSP90α expression. Levels of seromucoid, C-reactive protein, TNF-α, IL-1β and IL-10 were determined in blood serum.
Oral exposure to VNPs was associated with neither elevation of inflammation markers in blood serum nor HSP90α overexpression in the small intestine, i.e. no toxic effects of VNPs were observed. Carrageenan-induced intestinal inflammation was accompanied by higher levels of TNF-α and IL-1β, as well as HSP90α upregulation in the intestinal mucosa, compared with controls. Administration of VNPs to rats with enteritis did not lead to statistically significant changes in concentrations of circulating pro-inflammatory cytokines with the trend towards their increase.
No adverse effects were observed in rats orally exposed to VNPs at a dose of 20 μg/kg during two weeks. Using the experimental model of carrageenan-induced enteritis, it was demonstrated that VNPs at the dose used in our study did not affect the course of intestinal inflammation.
评估口服正钒酸钆（GdVO4:Eu3+）纳米粒子（VNPs）对慢性角叉菜胶诱导的肠道炎症病程的影响。
从小肠组织样本中收集四组大鼠（完整、给予 VNPs 后、角叉菜胶诱导的肠道炎症、角叉菜胶诱导的肠道炎症口服暴露于 VNPs），以评估肠道形态和 HSP90α 的表达。血清中测定黏蛋白、C 反应蛋白、TNF-α、IL-1β 和 IL-10 的水平。
口服暴露于 VNPs 既不会引起血清中炎症标志物的升高，也不会引起小肠中 HSP90α 的过度表达，即未观察到 VNPs 的毒性作用。与对照组相比，角叉菜胶诱导的肠道炎症导致 TNF-α 和 IL-1β 水平升高，以及肠黏膜中 HSP90α 的上调。给予肠炎大鼠 VNPs 不会导致循环促炎细胞因子浓度发生统计学显著变化，但有增加的趋势。
在两周内，以 20μg/kg 的剂量口服暴露于 VNPs 的大鼠未观察到不良反应。使用角叉菜胶诱导的肠炎实验模型，证明了在本研究中使用的剂量的 VNPs 不会影响肠道炎症的病程。
