流行出血病病毒的鸡胚传代模型。

An Embryonated Egg Transmission Model for Epizootic Hemorrhagic Disease Virus.

机构信息

Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.

出版信息

Vector Borne Zoonotic Dis. 2020 Sep;20(9):728-730. doi: 10.1089/vbz.2019.2590. Epub 2020 May 18.

DOI:10.1089/vbz.2019.2590

PMID:32423316

Abstract

Epizootic hemorrhagic disease virus (EHDV) is a vector-borne orbivirus of ruminants; in North America there are three serotypes (EHDV-1, -2, and -6) and these primarily affect white-tailed deer (). EHDV is vectored by biting midges, spp. Embryonated chicken eggs (ECE) have recently been used as an experimental host to investigate the vector competence of Australian spp. for bluetongue serotype virus 1 and 23. In this study, we evaluated the use of the ECE model to determine its applicability for evaluating vector competence related to transmission of North American EHDV serotypes. We demonstrated that all three North American EHDV serotypes were able to replicate in ECEs and be transmitted from infected ECEs to Wirth & Jones. In addition, we were able to complete the transmission cycle from infected to uninfected ECEs for EHDV-1 and -2.

摘要

鹿流行性出血热病毒（EHDV）是一种反刍动物的虫媒呼肠孤病毒；在北美洲，有三种血清型（EHDV-1、-2 和-6），主要影响白尾鹿（）。EHDV 由吸血蠓传播，如属。最近，鸡胚（ECE）已被用作实验宿主，以研究澳大利亚属对蓝舌病血清型 1 和 23 的媒介能力。在这项研究中，我们评估了使用 ECE 模型来确定其适用于评估与北美 EHDV 血清型传播相关的媒介能力的适用性。我们证明了所有三种北美 EHDV 血清型都能够在 ECE 中复制，并能够从感染的 ECE 传播到 Wirth & Jones。此外，我们能够完成从感染的到未感染的 ECE 的 EHDV-1 和 -2 的传播周期。