从体外到体内的神经转分化重编程：一种利用干细胞技术进行中枢神经系统组织重塑的方法。
From in vitro to in vivo reprogramming for neural transdifferentiation: An approach for CNS tissue remodeling using stem cell technology.
机构信息
Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
iPSC-based Drug Discovery and Development Team, RIKEN BioResource Research Center (BRC), Kyoto, Japan.
出版信息
J Cereb Blood Flow Metab. 2020 Sep;40(9):1739-1751. doi: 10.1177/0271678X20910324. Epub 2020 May 19.
Abstract
Advances in stem cell technology have provided three approaches to address the demanding issue of the treatment of intractable neurological disease. One of the approaches is the screening of compounds attenuating pathological phenotypes in stem-cell based models. A second approach consists of exogenous-targeted cell supplementation to the lesion with stem cell-derived differentiated cells. A third approach involves in vivo direct programming to transdifferentiate endogenous somatic cells and to boost CNS tissue remodeling. In this review, we outline research advances in stem cell technology of direct reprogramming in vitro and in vivo and discuss the future challenge of tissue remodeling by neural transdifferentiation.
摘要
干细胞技术的进步为解决难治性神经疾病的治疗这一难题提供了三种方法。一种方法是筛选减轻基于干细胞模型的病理表型的化合物。第二种方法包括用干细胞衍生的分化细胞对外源性靶向细胞进行补充，以修复病变。第三种方法涉及体内直接编程，使内源性体细胞转分化，并促进中枢神经系统组织重塑。在这篇综述中，我们概述了体外和体内直接重编程的干细胞技术的研究进展，并讨论了通过神经转分化进行组织重塑的未来挑战。
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