Ophthalmology Department, Hospital Universitario Fundacion Jimenez Diaz, Madrid, Spain
Rheumatology Department, Hospital Universitario Rey Juan Carlos, Mostoles, Madrid, Spain.
Br J Ophthalmol. 2021 Mar;105(3):322-327. doi: 10.1136/bjophthalmol-2019-315511. Epub 2020 May 18.
The aim of this study was to describe the cellular infiltrate in aqueous and vitreous samples of patients with uveitis analysed by multiparametric flow cytometry.
This is a retrospective analysis of aqueous and vitreous samples analysed by flow cytometry for diagnostic purposes, in cases of masquerade syndromes and infectious and non-infectious uveitis. Data collected included demographics, anatomical classification of uveitis, phenotypic diagnosis, anterior chamber cells grading, vitreous haze and time of follow-up since presentation to sample obtained.
Thirty-one samples (17 aqueous and 14 vitreous fluids) from 31 patients, 18 men, were analysed. The mean age at the time of sample collection was 60.23±17.03 years. The most frequent anatomical classification was panuveitis (14 of 31). T cells accounted for the main cellular component in the majority of the samples (10 of 13 aqueous samples; 7 of 14 in vitreous samples). CD4:CD8 ratios ranged from 0.21 to 16.3 in the case of aqueous samples and from 0.5 to 9.7 in the case of vitreous samples.
Flow cytometry analysis of aqueous and vitreous samples from patients with uveitis could provide insight into the pathogenesis of human uveitis and help develop accurate animal models which better mimic human disease.
本研究旨在描述通过多参数流式细胞术分析葡萄膜炎患者房水和玻璃体液样本中的细胞浸润情况。
这是一项回顾性分析，对用于诊断目的的房水和玻璃体液样本进行流式细胞术分析，包括伪装综合征、感染性和非感染性葡萄膜炎病例。收集的数据包括人口统计学、葡萄膜炎的解剖分类、表型诊断、前房细胞分级、玻璃体内混浊和自采集样本以来的随访时间。
对 31 名患者的 31 份样本（17 份房水样本和 14 份玻璃体液样本）进行了分析，其中男性 18 名，女性 13 名。采集样本时的平均年龄为 60.23±17.03 岁。最常见的解剖分类是全葡萄膜炎（31 例中的 14 例）。T 细胞是大多数样本中主要的细胞成分（13 份房水样本中的 10 份；14 份玻璃体液样本中的 7 份）。房水样本中 CD4:CD8 比值范围为 0.21 至 16.3，玻璃体液样本中 CD4:CD8 比值范围为 0.5 至 9.7。
对葡萄膜炎患者的房水和玻璃体液样本进行流式细胞术分析，可以深入了解人类葡萄膜炎的发病机制，并有助于开发出更好地模拟人类疾病的准确动物模型。
