Taguchi C, Sugita S, Tagawa Y, Nishihira J, Mochizuki M
Department of Ophthalmology, Kurume University School of Medicine, Fukuoka, Japan.
Br J Ophthalmol. 2001 Nov;85(11):1367-71. doi: 10.1136/bjo.85.11.1367.
To investigate the levels of macrophage migration inhibitory factor (MIF) in intraocular fluids of uveitis patients, the capacity of intraocular infiltrating lymphocytes to produce MIF, and the correlation between MIF levels in the eye and intraocular inflammatory activity.
MIF levels were measured by enzyme linked immunosorbent assay (ELISA) using (1) aqueous humour (AH) of 12 uveitis patients and eight control patients with cataract, (2) vitreous fluid of 15 uveitis patients and eight control patients with idiopathic macular hole, and (3) culture supernatants of T cell clones (TCCs) established from intraocular fluids of uveitis patients. MIF expression on infiltrating cells was determined by a double staining immunofluorescence technique using a flow cytometry.
Significant levels of MIF were detected from intraocular fluids of uveitis patients (4.0 (SD 3.0) ng/ml in AH and 16.5 (24.7) ng/ml in vitreous), whereas MIF levels in control groups were below the detectable levels. There was a significant correlation between MIF levels and vitreous inflammation (29.7 (30.0) ng/ml in active uveitis v 3.3 (2.6) ng/ml in inactive uveitis, p< 0.05). Significant levels of MIF were detected in culture supernatants of TCCs from ocular fluids of uveitis patients. MIF was expressed on infiltrating CD4+ lymphocytes from vitreous of uveitis patients.
Significant levels of MIF are present in intraocular fluids of patients with uveitis. Lymphocytes infiltrating in the eye are capable of producing MIF. MIF levels in vitreous fluid are correlated with vitreous inflammation activity. These data thus indicate that MIF in the eye has a significant role in the pathophysiology of ocular inflammation.
研究葡萄膜炎患者眼内液中巨噬细胞移动抑制因子（MIF）的水平、眼内浸润淋巴细胞产生MIF的能力，以及眼内MIF水平与眼内炎症活动之间的相关性。
采用酶联免疫吸附测定（ELISA）法检测MIF水平，具体如下：（1）12例葡萄膜炎患者和8例白内障对照患者的房水（AH）；（2）15例葡萄膜炎患者和8例特发性黄斑裂孔对照患者的玻璃体；（3）从葡萄膜炎患者眼内液中建立的T细胞克隆（TCCs）的培养上清液。采用双染色免疫荧光技术结合流式细胞术测定浸润细胞上的MIF表达。
在葡萄膜炎患者的眼内液中检测到显著水平的MIF（AH中为4.0（标准差3.0）ng/ml，玻璃体中为16.5（24.7）ng/ml），而对照组中的MIF水平低于可检测水平。MIF水平与玻璃体炎症之间存在显著相关性（活动性葡萄膜炎中为29.7（30.0）ng/ml，非活动性葡萄膜炎中为3.3（2.6）ng/ml，p<0.05）。在葡萄膜炎患者眼液来源的TCCs培养上清液中检测到显著水平的MIF。MIF在葡萄膜炎患者玻璃体浸润的CD4+淋巴细胞上表达。
葡萄膜炎患者的眼内液中存在显著水平的MIF。眼内浸润的淋巴细胞能够产生MIF。玻璃体中的MIF水平与玻璃体炎症活动相关。因此，这些数据表明眼内的MIF在眼部炎症的病理生理学中具有重要作用。
