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首次在 和 中检测到 。

First molecular detection of in and .

机构信息

College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60607, USA.

出版信息

Open Vet J. 2020 Apr;10(1):39-43. doi: 10.4314/ovj.v10i1.7. Epub 2020 Feb 22.

DOI:10.4314/ovj.v10i1.7
PMID:32426255
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7193872/
Abstract

BACKGROUND

is one of the most important avian malaria species, which is mainly seen in wild birds, with infections reported in more than 70 different species and at high prevalence.

AIM

The aim of this study was to determine the molecular prevalence of spp. in mosquitoes collected in China.

METHOD

A -specific fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) was established in this study to analyze five species of mosquitoes (1,620 pallens, 806 , 377 , 168 , and 80 ) collected in hand nets from homes in 25 provinces of China.

RESULTS

Only females originated from six provinces were determined to be positive (0.6%, 10/1,809). species were detected in three mosquito species, such as pallens (0.5%, 8/1,620), (0.6%, 1/168), and (0.3%, 1/377). Of the three mosquito species positive for , only pallens is known to feed on birds and is recognized as the natural vector of .

CONCLUSION

This is the first time that has been detected in and . , the agent of avian malaria, was present in mosquitoes in China, including mosquito species not previously thought to be the vectors.

摘要

背景

是最重要的禽疟物种之一,主要见于野生鸟类,已有超过 70 种不同的物种和高流行率的感染报告。

目的

本研究旨在确定在中国采集的蚊子中 spp. 的分子流行率。

方法

本研究建立了一种针对 spp. 的 -特异性荧光共振能量转移(FRET)聚合酶链反应(PCR),以分析来自中国 25 个省家庭用手网采集的 5 种蚊子(1,620 只 pallens、806 只 、377 只 、168 只 和 80 只 )。

结果

仅来自六个省份的雌性被确定为阳性(0.6%,10/1,809)。在三种蚊子中检测到了 种,如 pallens(0.5%,8/1,620)、 (0.6%,1/168)和 (0.3%,1/377)。在三种阳性蚊子中,只有 pallens 已知以鸟类为食,被认为是 的天然媒介。

结论

这是首次在中国的 和 中检测到 。禽疟的媒介物 存在于中国的蚊子中,包括以前认为不是媒介物的蚊子种类。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/984b/7193872/67527b7ab539/OpenVetJ-10-39-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/984b/7193872/67527b7ab539/OpenVetJ-10-39-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/984b/7193872/67527b7ab539/OpenVetJ-10-39-g001.jpg

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