Why orthoptera fauna resist of pesticide? First experimental data of resistance phenomena.

Orthoptera are capable of threat of agriculture, human health and resists to all pesticides used. This problem is become an objectif of many research's. Pesticide resistance is the adaptation of insects to this materials resulting in decreased susceptibility to that chemical. In other hand, insects develop a resistance through natural selection such chemically transformation, physiological phenomena and genetic. In our study, natural chitin was extracted from cuticle of orthoptera insect (southern of Algeria) using a chemical strategy consists on hydrochloric acid, sodium hydroxide and hydrogen peroxide. The average yield of extracted chitin (96.95% w) indicates that the cuticles of orthoptera are a rich source of chitin. Cuticle exhibit a heterogeneous morphology characterized by a compact structure with well-defined fibrous. For extracted chitin and after demineralization, we can appreciate important changes in the surface of material. We observed round shaped black spots indicated that they are composed almost exclusively by KO and CaO (cuticle) in the other hand we observed several white taches behind black spots, here we suggest that white taches present chitin extracted. The most resistant orthoptera are the ones to survive and transform their properties by chemical process such as transformation of chitin to chitosan and physiological development such as age. In this study, we have found that the first generation has a great resistance to insecticides. After insecticide application we observed a descendant's resistance decreased a larger because sensitive insects have been selectively killed. After repeated applications resistant insects may comprise the minority. Finally we can said, insecticide resistance can be found in many types and we can conclude that physiological resistance and chemical resistance coexist together and cannot separate. In the physiological resistance, the insect populations may develop the ability to avoid or reduce lethal insecticide exposure. In contrast, chemical resistance refers to modification mechanisms, including reduced cuticle penetration and decreased or increased target site sensitivity. The extracted chitin sample and chitosan were characterized by several characterizations such as X-ray diffraction, scanning electron microscopy SEM, FTIR and HRMN spectroscopy.