Yang Guang, Fang Jugao, Shi Qiang, Wang Ru, Lian Meng, Ma Hongzhi, Feng Ling, Shen Xixi, Wang Yu
Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical Universit, Beijing, 100730, China.
Department of Otorhinolaryngology Head and Neck Surgery, Former 263 ClinicalCenter of People's Liberation ArmyGeneral Hospital, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 100730, China.
World J Otorhinolaryngol Head Neck Surg. 2020 Jan 24;6(1):34-40. doi: 10.1016/j.wjorl.2019.05.001. eCollection 2020 Mar.
To investigate the expressions of MAPK10, c-Jun and Itga6 in laryngeal carcinoma and its influence on the sensitivity to docetaxel, cisplatin and 5-fluorouracil (TPF) chemotherapy.
Fifty-seven patients with supraglottic squamous cell carcinoma, who were treated by two cycles of TPF induction chemotherapy in our hospital, were enrolled in this study and divided into groups by chemotherapy resistance or chemotherapy sensitivity. The expressions of mRNA and protein of MAPK10, c-Jun and Itga6 in tumor tissues were evaluated by immunohistochemistry. The consistency of mRNA and protein expressions was tested, and the relation with the clinicopathological features was analyzed.
The positive rates of MAPK10 andc-Jun in the tumor tissues of the sensitive group were significantly higher than those of there assistant group, which was 90.48% and 100.00%, respectively. The expression rate of Itga6 was significantly higher in the resistant group, which was 83.33% ( < 0.05). The mRNA levels of MAPK10 and c-Jun were significantly lower in the resistant group than in the sensitive group, whilethemRNA levelof Itga6was significantly higher in the resistant group ( < 0.05). The protein expressions of MAPK10, c-Jun and Itga6 were consistent with their mRNA expressions ( < 0.05). The expressions of MAPK10, c-Jun and Itga6 were not correlatedwithage, gender and tumor diameter ( > 0.05). However, the expressions of MAPK10 and c-Jun were negatively correlated withclinical stage and pathological grading ( < 0.05). Negative correlations between MAPK 10 and Itga6, and between c-Jun and Itga6in tumor tissues were found by Spearman'srank correlation coefficient ( < 0.05). The correlation was also negative in the resistant tumor tissues ( < 0.05).
The MAPK10 and c-Jun expressions were down-regulated, while the Itga6 expression was up-regulated in the chemo-resistant laryngeal carcinoma, and the expression levels of different factors were correlated witheach other. These factorsmight be important biomarkers for predicting outcomes of TPF chemotherapy in laryngeal carcinoma in the future.
探讨丝裂原活化蛋白激酶10（MAPK10）、c-Jun和整合素α6（Itga6）在喉癌中的表达及其对多西他赛、顺铂和5-氟尿嘧啶（TPF）化疗敏感性的影响。
选取57例在我院接受2周期TPF诱导化疗的声门上型鳞状细胞癌患者，根据化疗耐药或化疗敏感分组。采用免疫组织化学法评估肿瘤组织中MAPK10、c-Jun和Itga6的mRNA和蛋白表达。检测mRNA和蛋白表达的一致性，并分析其与临床病理特征的关系。
敏感组肿瘤组织中MAPK10和c-Jun的阳性率显著高于耐药组，分别为90.48%和100.00%。Itga6在耐药组中的表达率显著更高，为83.33%（P<0.05）。耐药组中MAPK10和c-Jun的mRNA水平显著低于敏感组，而Itga6的mRNA水平在耐药组中显著更高（P<0.05）。MAPK10、c-Jun和Itga6的蛋白表达与其mRNA表达一致（P<0.05）。MAPK10、c-Jun和Itga6的表达与年龄、性别和肿瘤直径无关（P>0.05）。然而，MAPK10和c-Jun的表达与临床分期和病理分级呈负相关（P<0.05）。通过Spearman等级相关系数发现肿瘤组织中MAPK10与Itga6之间以及c-Jun与Itga6之间呈负相关（P<0.05）。在耐药肿瘤组织中相关性也为负（P<0.05）。
化疗耐药的喉癌中MAPK10和c-Jun表达下调，而Itga6表达上调，且不同因子的表达水平相互关联。这些因子可能是未来预测喉癌TPF化疗疗效的重要生物标志物。
