用²⁵²Cf等离子体解吸质谱法对正常和修饰的寡核苷酸进行表征和测序。

Characterization and sequencing of normal and modified oligonucleotides by 252Cf plasma desorption mass spectrometry.

作者信息

Viari A, Ballini J P, Meleard P, Vigny P, Dousset P, Blonski C, Shire D

机构信息

Laboratoire de Physique et Chimie Biomoléculaire (CNRS UA 198), Institut Curie et Université Paris VI, France.

出版信息

Biomed Environ Mass Spectrom. 1988 Oct;16(1-12):225-8. doi: 10.1002/bms.1200160141.

DOI:10.1002/bms.1200160141

PMID:3242675

Abstract

Plasma desorption (PD) mass spectra of normal deoxyribo-oligonucleotides and of neutral methylphosphonate deoxyribo-oligonucleosides are examined and discussed. Molecular ions of oligonucleotides up to nonamer have been observed for neutral species. It is also shown that PD mass spectra can be used to monitor chemical modifications of oligonucleotides, such as the covalent binding of an organic fluorescent probe, along the synthesis process.

摘要

对正常脱氧核糖寡核苷酸和中性甲基膦酸酯脱氧核糖寡核苷的等离子体解吸（PD）质谱进行了研究和讨论。已观察到中性物种中直至九聚体的寡核苷酸分子离子。研究还表明，PD质谱可用于监测寡核苷酸在合成过程中的化学修饰，例如有机荧光探针的共价结合。