Jurinke Christian, Oeth Paul, van den Boom Dirk
Sequenom, Inc., Johns Hopkins Court, San Diego, CA 92121, USA.
Mol Biotechnol. 2004 Feb;26(2):147-64. doi: 10.1385/MB:26:2:147.
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field of nucleic acid analysis. These include the analysis of mutations, the resequencing of amplicons with a known reference sequence, and the quantitative analysis of gene expression and allelic frequencies in complex DNA mixtures.
基质辅助激光解吸/电离（MALDI）飞行时间（TOF）质谱（MS）在过去十年中已发展成为一种用于生物聚合物分析的多功能工具。本综述的目的是总结这一发展，并概述已在核酸分析领域得以常规应用的相关技术。这些技术包括突变分析、对具有已知参考序列的扩增子进行重测序，以及对复杂DNA混合物中的基因表达和等位基因频率进行定量分析。
